Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:80025
Wallis D, et al., The zinc finger transcription factor Gfi1, implicated in lymphomagenesis, is required for inner ear hair cell differentiation and survival. Development. 2003 Jan;130(1):221-32
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:5302552
|
|
Gene symbol:
|
Myo6
|
|
Gene name:
|
myosin VI
|
|
Antibody:
|
anti-MyoVI/VIIa
|
|
Detection system:
|
Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
|
|
Assay notes:
|
The antibody used in this assay recognizes two myosin isoforms; both have been coded.
|
|
|
Results
|
Specimen
3A:
embryonic day 13.5
(more  )
Specimen
3A:
(close  )
|
Genetic Background:
|
involves: 129S1/Sv * C57BL/6J
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: utricle epithelium |
Present |
Regionally restricted |
3A
|
Expression is present in the hair cells.
|
Specimen
3B:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * C57BL/6J
|
|
Mutant Allele(s):
|
Gfi1tm1Sho/Gfi1tm1Sho
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: utricle epithelium |
Present |
Regionally restricted |
3B
|
Expression is present in the hair cells. They are have abnormal morphology and layering in the mutant.
|
Specimen
3C:
embryonic day 14.5
(more )
Specimen
3C:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * C57BL/6J
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: utricle epithelium |
Present |
Regionally restricted |
3C
|
Expression is present in the hair cells.
|
Specimen
3D:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * C57BL/6J
|
|
Mutant Allele(s):
|
Gfi1tm1Sho/Gfi1tm1Sho
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: utricle epithelium |
Present |
Regionally restricted |
3D
|
Expression is present in the hair cells. They are have abnormal morphology and layering in the mutant.
|
Specimen
3E:
embryonic day 16.5
(more )
Specimen
3E:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * C57BL/6J
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: organ of Corti |
Present |
Regionally restricted |
3E
|
Expressing cells are present in the organ of Corti.
|
Specimen
3F:
(close )
|
Genetic Background:
|
involves: 129S1/Sv * C57BL/6J
|
|
Mutant Allele(s):
|
Gfi1tm1Sho/Gfi1tm1Sho
|
|
Age:
|
embryonic day 16.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS24: organ of Corti |
Present |
Regionally restricted |
3F
|
Expressing cells are present in the organ of Corti; in the mutant they are disorganized.
|
|
Analysis Tools