Gene Expression Data
Assay Details
Assay
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Reference:
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J:80025
Wallis D, et al., The zinc finger transcription factor Gfi1, implicated in lymphomagenesis, is required for inner ear hair cell differentiation and survival. Development. 2003 Jan;130(1):221-32
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5302551
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Gene symbol:
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Gfi1
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Gene name:
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growth factor independent 1 transcription repressor
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Antibody:
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Gfi1 antibody
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
1C:
embryonic day 16.5
(more )
Specimen
1C:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS24: retina |
Present |
Regionally restricted |
1C
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Expression is present in cells that are likely to be retinal ganglion cells.
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Specimen
1F:
embryonic day 18.5
(more )
Specimen
1F:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: skin |
Present |
Single cells |
1F
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Expressing cells are found around the shaft of the whiskers; they are thought to be Merkel cells.
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Specimen
1J:
embryonic day 16.5
(more )
Specimen
1J:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 16.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: lung |
Present |
Non-Uniform |
1J
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Expression is present in clusters of cells believed to be neuroepithelial precursor cells.
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Specimen
2E:
embryonic day 14.5
(more )
Specimen
2E:
(close )
Genetic Background:
|
Not Specified
|
Age:
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embryonic day 14.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS22: saccule |
Present |
Regionally restricted |
2E
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Expression is present in the developing vestibule organs in positions where the newly developed hair cells are believed to be.
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TS22: utricle |
Present |
Regionally restricted |
2E
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Expression is present in the developing vestibule organs in positions where the newly developed hair cells are believed to be.
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Specimen
2F:
embryonic day 18.5
(more )
Specimen
2F:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS26: organ of Corti |
Strong |
Regionally restricted |
2F
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High levels of expression can be seen in the hair cells.
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Specimen
4C:
embryonic day 12.5
(more )
Specimen
4C:
(close )
Genetic Background:
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involves: 129 * C57BL/6J
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
4D:
(close )
Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Atoh1tm2Hzo/Atoh1tm2Hzo
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
Not shown:
(close )
Genetic Background:
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involves: 129 * C57BL/6J
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Mutant Allele(s):
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Atoh1tm2Hzo/Atoh1tm2Hzo
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Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
Not shown:
(close )
Genetic Background:
|
involves: 129 * C57BL/6J
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Mutant Allele(s):
|
Atoh1tm2Hzo/Atoh1tm2Hzo
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Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
Not shown:
(close )
Genetic Background:
|
involves: 129 * C57BL/6J
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Mutant Allele(s):
|
Atoh1tm2Hzo/Atoh1tm2Hzo
|
Age:
|
embryonic day 18.5
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Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
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