Probe:	Gfi1 probe1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS20: dorsal root ganglion	Strong		1A	This is an area of high expression.
TS20: forebrain	Strong		1A	This is an area of high expression.
TS20: gut epithelium	Strong		1A	This is an area of high expression.
TS20: hindbrain	Strong		1A	This is an area of high expression.
TS20: eye	Strong		1A	There is high expression in the optic epithelium.
TS20: inner ear	Present		1A	Expression is present in the otic vesicle.
TS20: trigeminal ganglion	Present		1A
TS20: vestibulo-cochlear ganglion	Present		1A

Structure	Level	Pattern	Image	Note
TS26: retina	Present	Regionally restricted	1B	Expression is primarily present in the retinal ganglion cell layer.

Structure	Level	Pattern	Image	Note
TS24: skin	Present	Single cells	1D	Expression is present in cells around the shaft of whiskers; they are thought to be Merkel cells.

Structure	Level	Pattern	Image	Note
TS23: skin	Present	Single cells	1E	Expressing cells are located under the touch domes; they are believed to be Merkel cells.

Structure	Level	Pattern	Image	Note
TS24: olfactory epithelium	Present		1G

Structure	Level	Pattern	Image	Note
TS26: tongue epithelium	Present	Regionally restricted	1H	Expression is present in the dorsal eptihelium where taste papilla develop.

Structure	Level	Pattern	Image	Note
TS23: lung	Present	Non-Uniform	1I	Expression is present in clusters of cells believed to be neuroepithelial precursor cells.

Structure	Level	Pattern	Image	Note
TS23: thymus primordium	Present		1K

Structure	Level	Pattern	Image	Note
TS20: inner ear	Present		2A	Expression is present in the otic vesicle.
TS20: cranial ganglion	Present		2A	Expression is present in several ganglia.
TS20: vestibulo-cochlear ganglion	Present		2A

Structure	Level	Pattern	Image	Note
TS22: saccule	Present	Regionally restricted	2B	Expression is present in the saccule, but high levels of expression are not yet visible in the cochlea. Expression is present in positions where the newly developed hair cells are believed to be.
TS22: utricle	Present		2B	Expression is present in the developing vestibule organs in positions where the newly developed hair cells are believed to be.

Structure	Level	Pattern	Image	Note
TS24: cochlea	Present		2C
TS24: saccule	Present		2C
TS24: utricle	Present		2C

Structure	Level	Pattern	Image	Note
TS26: organ of Corti	Strong	Regionally restricted	2D	Expression can be seen in the hair cells.
TS26: cochlear ganglion	Present		2D

Structure	Level	Pattern	Image	Note
TS20: inner ear vestibular component	Present	Regionally restricted	4A	Expression is present in the entire otic epithelium.

Structure	Level	Pattern	Image	Note
TS20: inner ear vestibular component	Present	Regionally restricted	4B	As in wild type, expression is present in the entire otic epithelium.

Structure	Level	Pattern	Image	Note
TS26: cochlea epithelium	Present	Regionally restricted	4E	Expression is present in the hair cells.
TS26: saccule epithelium	Present	Regionally restricted	4E	Expression is present in the hair cells.
TS26: utricle epithelium	Present	Regionally restricted	4E	Expression is present in the hair cells.

Structure	Level	Pattern	Image	Note
TS26: cochlea	Absent		4F	Lack of expression is assumed to be due to the loss of hair cells in the mutant.
TS26: saccule	Absent		4F	Lack of expression is assumed to be due to the loss of hair cells in the mutant.
TS26: utricle	Absent		4F	Lack of expression is assumed to be due to the loss of hair cells in the mutant.