Gene Expression Data
Assay Details
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Assay
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Reference:
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J:57297
St-Jacques B, et al., Indian hedgehog signaling regulates proliferation and differentiation of chondrocytes and is essential for bone formation [published erratum appears in Genes Dev 1999 Oct 1;13(19):2617]. Genes Dev. 1999 Aug 15;13(16):2072-86
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5302038
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Gene symbol:
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Col10a1
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Gene name:
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collagen, type X, alpha 1
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Probe:
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type X collagen cDNA2
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
6A:
embryonic day 12.5
(more  )
Specimen
6A:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ihhtm1Amc/Ihhtm1Amc
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6C:
embryonic day 13.5
(more )
Specimen
6C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
6D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ihhtm1Amc/Ihhtm1Amc
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: humerus cartilage condensation |
Present |
Patchy |
6D
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Expression is present in hypertrophic chondrocytes. Chondrocyte maturation appears to be delayed in the mutants.
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Specimen
6F:
embryonic day 14.5
(more )
Specimen
6F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: humerus cartilage condensation |
Present |
Regionally restricted |
6F
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Expression is present in hypertrophic chondrocytes. They are in a region that is almost nonoverlapping with the region containing less mature chondrocytes.
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Specimen
6H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ihhtm1Amc/Ihhtm1Amc
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: humerus cartilage condensation |
Present |
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6H
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Expression is present in hypertrophic chondrocytes. Unlike in wild type, in the mutant they are in a region that broadly overlaps with the region containing less mature chondrocytes.
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Specimen
6J:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ihhtm1Amc/Ihhtm1Amc
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: fibula |
Present |
Regionally restricted |
6J
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Expression is present in mature chondrocytes in the center of the cartilage.
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Specimen
6L:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ
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Mutant Allele(s):
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Ihhtm1Amc/Ihhtm1Amc
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: humerus |
Present |
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6L
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Expression is present in the mutant, but cells in the center of the humerus do not express, although most of these cells have the appearance of hypertrophic chondrocytes.
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Analysis Tools