Probe:	Isl1 cDNA7
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS17: rhombomere	Present	Non-Uniform	3A	Expression is detected in the ventral domain of all rhombomeres, except rhombomere 1. It was strongest in r2 and r4, corresponding to the precursors of the trigeminal and facial motoneurons.
TS17: rhombomere 2	Present	Non-Uniform	3A	Expression is detected in the ventral domain of all rhombomeres, except rhombomere 1. It was strongest in r2 and r4, corresponding to the precursors of the trigeminal and facial motoneurons.
TS17: rhombomere 4	Present	Non-Uniform	3A	Expression is detected in the ventral domain of all rhombomeres, except rhombomere 1. It was strongest in r2 and r4, corresponding to the precursors of the trigeminal and facial motoneurons.

Structure	Level	Pattern	Image	Note
TS17: rhombomere	Present	Non-Uniform	3B	Expression is detected in the ventral domain of all rhombomeres, except rhombomere 1. It was strongest in r2 and r4, corresponding to the precursors of the trigeminal and facial motoneurons.
TS17: rhombomere 2	Present	Non-Uniform	3B	Expression is detected in the ventral domain of all rhombomeres, except rhombomere 1. It was strongest in r2 and r4, corresponding to the precursors of the trigeminal and facial motoneurons.
TS17: rhombomere 4	Present	Non-Uniform	3B	Expression is detected in the ventral domain of all rhombomeres, except rhombomere 1. It was strongest in r2 and r4, corresponding to the precursors of the trigeminal and facial motoneurons.

Structure	Level	Pattern	Image	Note
TS18: hindbrain	Present	Non-Uniform	3C	Areas of expression include rhombomeres 2 and 4, in areas corresponding to the precursors of the trigeminal and facial motoneurons.
TS18: rhombomere 2	Present	Non-Uniform	3C	Areas of expression include rhombomeres 2 and 4, in areas corresponding to the precursors of the trigeminal and facial motoneurons.
TS18: rhombomere 4	Present	Non-Uniform	3C	Areas of expression include rhombomeres 2 and 4, in areas corresponding to the precursors of the trigeminal and facial motoneurons.
TS18: trigeminal ganglion	Present		3C
TS18: cranial ganglion	Present		3C	Expression is present in the migrating facial motoneurons.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Non-Uniform	3D	Areas of expression include rhombomeres 2 and 4, in areas corresponding to the precursors of the trigeminal and facial motoneurons.
TS19: facial ganglion	Present		3D
TS19: trigeminal ganglion	Present		3D

Structure	Level	Pattern	Image	Note
TS18: hindbrain	Present	Non-Uniform	3E	Expression is present in motoneurons.
TS18: trigeminal ganglion	Present		3E
TS18: cranial ganglion	Present		3E	Expression is present in the migrating facial motoneurons.

Structure	Level	Pattern	Image	Note
TS18: rhombomere 4	Present	Regionally restricted	3F	There are ectopic motor nuclei in rhombomere 4 in the mutant.
TS18: trigeminal ganglion	Present		3F
TS18: cranial ganglion	Present		3F	The facial motoneuron population is strongly reduced in the mutant.

Structure	Level	Pattern	Image	Note
TS18: rhombomere 4	Present	Regionally restricted	3G	There are ectopic motor nuclei in rhombomere 4 in the mutant.
TS18: trigeminal ganglion	Present		3G
TS18: cranial ganglion	Present	Regionally restricted	3G	Expression is present in the abducens motoneurons. The facial motoneuron population is absent in these mutants.

Structure	Level	Pattern	Image	Note
TS18: rhombomere 4	Present	Regionally restricted	3H	There are ectopic motor nuclei in rhombomere 4 in the mutant.
TS18: trigeminal ganglion	Present		3H
TS18: cranial ganglion	Present	Regionally restricted	3H	The facial motoneuron population is depleted in these mutants.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Non-Uniform	3I	Expression is present in motoneurons.
TS20: facial ganglion	Present		3I
TS20: trigeminal ganglion	Present		3I

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Non-Uniform	3J	Expression is present in motoneurons. Ectopic motor nuclei are formed in rhombomere 4. The abducens motoneurons could not be detected.
TS20: trigeminal ganglion	Present		3J
TS20: facial ganglion	Trace		3J	In the mutant there is sparce migration of the facial motoneurons; they turn dorsally prematurely.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Non-Uniform	3K	Expression is present in motoneurons. Ectopic motor nuclei are formed in rhombomere 4.
TS20: trigeminal ganglion	Present		3K
TS20: cranial ganglion	Present	Regionally restricted	3K	There is expression in the abducens motoneurons.
TS20: facial ganglion	Absent		3K	In the mutants the facial motoneurons do not follow the normal migration pathway.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Non-Uniform	3L	Expression is present in motoneurons. Ectopic motor nuclei are formed in rhombomere 4.
TS20: trigeminal ganglion	Present		3L
TS20: facial ganglion	Present		3L	In the mutants the facial motoneuron population is reduced.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Non-Uniform	3M	Expression is present in motoneurons.
TS20: facial ganglion	Present		3M
TS20: trigeminal ganglion	Present		3M

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Non-Uniform	3N	Expression is present in motoneurons. Ectopic motor nuclei are formed in rhombomere 4. The abducens motoneurons could not be detected.
TS20: trigeminal ganglion	Present		3N
TS20: facial ganglion	Absent		3N	In the mutant there is sparce migration of the facial motoneurons; they turn dorsally prematurely.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Non-Uniform	3O	Expression is present in motoneurons. Ectopic motor nuclei are formed in rhombomere 4.
TS20: trigeminal ganglion	Present		3O
TS20: cranial ganglion	Present		3O	Expression is present in the abducens motoneurons.
TS20: facial ganglion	Absent		3O	In the mutants the facial motoneurons do not follow the normal migration pathway.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Non-Uniform	3P	Expression is present in motoneurons. Ectopic motor nuclei are formed in rhombomere 4.
TS20: trigeminal ganglion	Present		3P
TS20: facial ganglion	Present		3P	In the mutants the facial motoneuron population follows the normal migratory pathway, but its number is variably reduced.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Non-Uniform	3Q	Expression is present in motoneurons. The ectopic nuceli found in the mutants are variable in both their number (density) and position.
TS20: trigeminal ganglion	Present		3Q
TS20: facial ganglion	Present		3Q	In the mutants the facial motoneuron population following the normal migratory pathway is variably reduced. This reduction is inversely correlated with the number and size of the ectopic nuclei found elsewhere in the hindbrain.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Non-Uniform	3R	Expression is present in motoneurons. The ectopic nuceli found in the mutants are variable in both their number (density) and position.
TS20: trigeminal ganglion	Present		3R
TS20: facial ganglion	Present		3R	In the mutants the facial motoneuron population following the normal migratory pathway is variably reduced. This reduction is inversely correlated with the number and size of the ectopic nuclei found elsewhere in the hindbrain.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Non-Uniform	3S	Expression is present in motoneurons. The ectopic nuceli found in the mutants are variable in both their number (density) and position.
TS20: trigeminal ganglion	Present		3S
TS20: facial ganglion	Present		3S	In the mutants the facial motoneuron population following the normal migratory pathway is variably reduced. This reduction is inversely correlated with the number and size of the ectopic nuclei found elsewhere in the hindbrain.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Non-Uniform	3T	Expression is present in motoneurons. The ectopic nuceli found in the mutants are variable in both their number (density) and position.
TS20: trigeminal ganglion	Present		3T
TS20: facial ganglion	Present		3T	In the mutants the facial motoneuron population following the normal migratory pathway is reduced and its behavior variable.

Structure	Level	Pattern	Image	Note
TS21: facial ganglion	Present		7C
TS21: trigeminal ganglion	Present		7C
TS21: cranial ganglion	Present	Regionally restricted	7C	Expression is present in the abducens motoneurons.

Structure	Level	Pattern	Image	Note
TS21: facial ganglion	Present		7D	The nucleus is of variable size and present in most cases.
TS21: cranial ganglion	Present	Regionally restricted	7D	Expression is present in the facial motoneurons, but the abducens motoneurons cannot be detected. There are also no ectopic expressing nuclei.
TS21: trigeminal ganglion	Present		7D

Structure	Level	Pattern	Image	Note
TS21: cranial ganglion	Present	Regionally restricted	7E	Expression is present in the abducens motoneurons, as well as in ectopic nuclei.
TS21: facial ganglion	Absent		7E
TS21: trigeminal ganglion	Present		7E

Structure	Level	Pattern	Image	Note
TS21: cranial ganglion	Present	Regionally restricted	7F	Expression is present in the abducens motoneurons, as well as in ectopic nuclei.
TS21: facial ganglion	Present		7F	The nucleus is smaller in the mutant compared to wild type.
TS21: trigeminal ganglion	Present		7F

Structure	Level	Pattern	Image	Note
TS22: facial ganglion	Present		7G
TS22: trigeminal ganglion	Present		7G
TS22: cranial ganglion	Present	Regionally restricted	7G	Expression is present in the abducens motoneurons.

Structure	Level	Pattern	Image	Note
TS22: facial ganglion	Present		7H	The nucleus is of variable size and present in most cases.
TS22: cranial ganglion	Present	Regionally restricted	7H	Expression is present in the facial motoneurons, but the abducens motoneurons cannot be detected. There are also no ectopic expressing nuclei.
TS22: trigeminal ganglion	Present		7H

Structure	Level	Pattern	Image	Note
TS22: cranial ganglion	Present		7I	Expression is present in the abducens motoneurons. It is no longer present in ectopic nuclei.
TS22: facial ganglion	Absent		7I
TS22: trigeminal ganglion	Present		7I

Structure	Level	Pattern	Image	Note
TS22: cranial ganglion	Present	Regionally restricted	7J	Expression is present in the abducens motoneurons. It is no longer present in ectopic nuclei.
TS22: facial ganglion	Present	Regionally restricted	7J	The nucleus is reduced in the mutant compared to wild type.
TS22: trigeminal ganglion	Present		7J

Structure	Level	Pattern	Image	Note
TS22: facial ganglion	Present		8A
TS22: trigeminal ganglion	Present		8A
TS22: cranial ganglion	Present	Regionally restricted	8A	Expression is present in the abducens motoneurons.

Structure	Level	Pattern	Image	Note
TS22: facial ganglion	Present		8B
TS22: trigeminal ganglion	Present		8B
TS22: cranial ganglion	Present	Regionally restricted	8B	Expression is present in the abducens motoneurons.
TS22: hindbrain	Present	Non-Uniform	8B	In the mutant there is an additional ventral column of ectopic expressing cells in the caudal hindbrain.

Structure	Level	Pattern	Image	Note
TS22: trigeminal ganglion	Present		8C
TS22: cranial ganglion	Present	Regionally restricted	8C	Expression is present in the abducens motoneurons.
TS22: facial ganglion	Absent		8C

Structure	Level	Pattern	Image	Note
TS22: trigeminal ganglion	Present		8D
TS22: cranial ganglion	Present	Regionally restricted	8D	Expression is present in the abducens motoneurons.
TS22: facial ganglion	Absent		8D
TS22: hindbrain	Present	Regionally restricted	8D	In the mutant there is an additional ventral column of ectopic expressing cells in the caudal hindbrain.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Non-Uniform		In the mutant areas of expression include a ventral column of ectopic expressing cells in the caudal hindbrain.