Probe:	Math3 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Weak	Non-Uniform	2A	There is expression in a broad ventral domain throughout the hindbrain; there is higher expression in a narrow stripe adjacent to the floorplate.
TS17: rhombomere 4	Present	Non-Uniform	2A	Expression is particularly strong in a ventral rhombomere 4 column. It is also present in a medial column extending from rhombomere 4-6 and in a dorsal column extending from rhombomere 4 caudally.
TS17: rhombomere 5	Present	Non-Uniform	2A	Expression is present in a medial column extending from rhombomere 4-6 and in a dorsal column extending from rhombomere 4 caudally.
TS17: rhombomere 6	Present	Non-Uniform	2A	Expression is present in a medial column extending from rhombomere 4-6 and in a dorsal column extending from rhombomere 4 caudally.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Non-Uniform	2B	Expression within the dorsal column extends throughout only two rhombomeres.
TS17: rhombomere 4	Present		2B	There is no rhombomere 4 specific upregulation of the ventral column of the mutant, leaving expression similar to that seen in rhombomeres 2 and 3. Expression within the dorsal column extends throughout only two rhombomeres.
TS17: rhombomere 2	Present		2B
TS17: rhombomere 3	Present		2B

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Non-Uniform	2C	Expression in the dorsal column appears normal.
TS17: rhombomere 4	Present	Non-Uniform	2C	Relative to wild type, the expression in the ventral domain is reduced but it is still stronger than that seen in rhombomeres 3 or 5.
TS17: rhombomere 3	Present		2C
TS17: rhombomere 5	Present		2C

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Non-Uniform	2D	Expression is present in the mutant but, unlike in wild-type, there is no rhombomere 4 specific upregulation of the ventral column. Expression within the dorsal column extends throughout only one rhombomere.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Non-Uniform	2E	Expression is resolved into longitudinal stripes; it is particularly strong in two stripes in ventral rhombomere 4 and in a medial column extending from rhombomere 4-6. There is also some upregulation in a more dorsal column extending caudally from r4.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Non-Uniform	2F	Expression is present in the mutant but, unlike in wild-type, there is no rhombomere 4 specific upregulation of the ventral column. Expression within the dorsal column extended throughout only two rhombomeres.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Non-Uniform	2G	Expression is present in the mutant but, unlike in wild-type, there is no rhombomere 4 specific upregulation of the ventral column. Instead expression is variable in this domain.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Non-Uniform	2H	Expression is present in the mutant but, unlike in wild-type, there is no rhombomere 4 specific upregulation of the ventral column. Expression within the dorsal column extends throughout only one rhombomere.