Gene Expression Data
Assay Details
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Assay
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Reference:
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J:86049
Gavalas A, et al., Neuronal defects in the hindbrain of Hoxa1, Hoxb1 and Hoxb2 mutants reflect regulatory interactions among these Hox genes. Development. 2003 Dec;130(23):5663-79
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5300083
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Gene symbol:
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Hoxb1
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Gene name:
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homeobox B1
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Probe:
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Hoxb1 cDNA3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
1A:
embryonic day 10.5
(more  )
Note: The specimen is flat-mounted.
Specimen
1A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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The specimen is flat-mounted.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: rhombomere 4 |
Present |
Non-Uniform |
1A
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Expression is upregulated in ventral and dorsal columns.
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Specimen
1B:
embryonic day 11.5
(more )
Note: The specimen is flat-mounted.
Specimen
1B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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The specimen is flat-mounted.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindbrain |
Present |
Regionally restricted |
1B
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Expression is restricted to rhombomere 4; it is upregulated in ventral and dorsal columns. The ventral expression is further subdivided into two adjacent stripes--one next to the floorplate and a broader one that curves away from the midline.
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Specimen
1C:
embryonic day 12.5
(more )
Note: The specimen is flat-mounted.
Specimen
1C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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The specimen is flat-mounted.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: hindbrain |
Present |
Regionally restricted |
1C
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Expression is restricted to rhombomere 4, although it is generally downregulated from what was seen earlier. It is upregulated in ventral and dorsal columns.
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Specimen
9A:
embryonic day 8.5
(more )
Specimen
9A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
9B:
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Hoxb2tm1Fmr/Hoxb2tm1Fmr
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
9C:
embryonic day 9.0
(more )
Specimen
9C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
9D:
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Hoxb2tm1Fmr/Hoxb2tm1Fmr
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Age:
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embryonic day 9.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: rhombomere 4 |
Weak |
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9D
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Expression is not properly maintained in the mutant.
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Specimen
9E:
embryonic day 10.5
(more )
Specimen
9E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
9F:
(close )
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Genetic Background:
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involves: 129
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Mutant Allele(s):
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Hoxb2tm1Fmr/Hoxb2tm1Fmr
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: rhombomere 4 |
Weak |
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9F
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Expression is not properly maintained in the mutant.
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