Gene Expression Data
Assay Details
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Assay
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Reference:
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J:75933
Clotman F, et al., The onecut transcription factor HNF6 is required for normal development of the biliary tract. Development. 2002 Apr;129(8):1819-28
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5298844
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Gene symbol:
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Hnf1b
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Gene name:
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HNF1 homeobox B
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Antibody:
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anti-HNF1beta
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Assay notes:
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Dissected fetuses or livers were directly frozen in liquid nitrogen cooled
isopentane or fixed in formaldehyde [1% in phosphatebuffered
saline (PBS) for 2 hours], embedded in gelatin/sucrose. Sections of
fresh-frozen tissue were post-fixed in acetone.
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Results
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Specimen
6A:
embryonic day 15.5
(more  )
Specimen
6A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: liver |
Strong |
Regionally restricted |
6A
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Expression is in the ductal plate, in cells located at the interface between the portal mesenchyme and the liver parenchyma
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Specimen
6A inset:
embryonic day 15.5
(more )
Specimen
6A inset:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: gallbladder |
Present |
Regionally restricted |
6A inset
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Expression is in the epithelium of the gallbladder primordium, but not in the surrounding mesenchyme.
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Specimen
6B:
embryonic day 15.5
(more )
Specimen
6B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: common bile duct |
Strong |
Regionally restricted |
6B
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Expression is in the epithelium.
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| TS23: duodenum |
Weak |
Regionally restricted |
6B
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Expression is in the epithelium.
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Specimen
6B inset:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Swiss
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Mutant Allele(s):
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Onecut1tm1Flem/Onecut1tm1Flem
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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Not Specified
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Fixation:
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Other - See Notes
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Embedding:
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Cryosection
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Analysis Tools