Gene Expression Data
Assay Details
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Assay
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Reference:
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J:52756
Moore AW, et al., YAC complementation shows a requirement for Wt1 in the development of epicardium, adrenal gland and throughout nephrogenesis. Development. 1999 May;126(9):1845-57
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5298345
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Gene symbol:
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Wt1
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Gene name:
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WT1 transcription factor
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Antibody:
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anti-WT1 (C-19; sc-192)
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Detection system:
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Secondary antibody coupled to fluorescein
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Assay notes:
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Antibody was either the C-19 or the H-2 (discontinued) antibody from Santa Cruz Biotechnology.
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Results
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Specimen
1D:
embryonic day 13.5
(more  )
Note: Double labeled: green - Wt1; red - Cytokeratins.
Specimen
1D:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Double labeled: green - Wt1; red - Cytokeratins.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: heart ventricle |
Present |
Regionally restricted |
1D
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Expression is in the epicardium and subepicardial mesenchyme of a ventricle.
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Specimen
1E:
embryonic day 13.5
(more )
Note: Double labeled: green - Wt1; red - Cytokeratins.
Specimen
1E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Double labeled: green - Wt1; red - Cytokeratins.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: heart |
Present |
Regionally restricted |
1E
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Expression is in the intraventricular sulcus.
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Specimen
5A:
embryonic day 17.5
(more )
Note: Double labeled: green - Wt1; red - Pax2 (assay MGI:5298363).
Specimen
5A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Double labeled: green - Wt1; red - Pax2 (assay MGI:5298363).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: metanephros |
Present |
Regionally restricted |
5A
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Expression is in the metanephric mesenchyme of the kidney periphery and in the condensing blastema.
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| TS25: comma-shaped body |
Present |
Regionally restricted |
5A
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Expression is in the proximal part of the comma-shaped bodies.
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| TS25: glomerular tuft |
Present |
Regionally restricted |
5A
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Expression is in podocytes.
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Specimen
5B:
embryonic day 17.5
(more )
Note: Double labeled: green - Wt1; red - Cytokeratins.
Specimen
5B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Double labeled: green - Wt1; red - Cytokeratins.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: metanephros |
Present |
Regionally restricted |
5B
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Expression is in the metanephric mesenchyme of the kidney periphery and in the condensing blastema.
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| TS25: comma-shaped body |
Present |
Regionally restricted |
5B
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Expression is in the proximal part of the comma-shaped bodies.
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| TS25: glomerular tuft |
Present |
Regionally restricted |
5B
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Expression is in podocytes.
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Specimen
5C:
embryonic day 17.5
(more )
Note: Double labeled: green - Wt1; red - Tnc (assay MGI:5298358).
Specimen
5C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Note:
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Double labeled: green - Wt1; red - Tnc (assay MGI:5298358).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: metanephros |
Present |
Regionally restricted |
5C
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Expression is in the metanephric mesenchyme of the kidney periphery and in the condensing blastema.
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| TS25: comma-shaped body |
Present |
Regionally restricted |
5C
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Expression is in the proximal part of the comma-shaped bodies.
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| TS25: glomerular tuft |
Present |
Regionally restricted |
5C
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Expression is in podocytes.
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Analysis Tools