Probe:	Wnt5a cDNA11
Probe preparation:	Antisense RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS28: decidua	Strong	Regionally restricted	6a/b	Expression is present at the mesometrial pole of the deciduum in the mesometrial component.

Structure	Level	Pattern	Image	Note
TS9: embryo ectoderm	Present	Regionally restricted		Expression is present in the posterior.
TS9: embryo mesoderm	Present	Regionally restricted		Expression is present in the posterior.
TS9: primary trophoblast giant cell	Present			Expression is present in trophectoderm giant cells.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present		6c/d	Expression is distributed throughout the neuroectoderm.
TS15: trunk mesenchyme	Present		6c/d	Expression is distributed throughout the mesoderm.
TS15: gut epithelium	Present		6c/d	Expression is distributed throughout the endodermal epithelium of the gut.

Structure	Level	Pattern	Image	Note
TS15: future forebrain	Absent
TS15: future hindbrain	Present	Regionally restricted		Expression is present in the caudal hindbrain/myelencephalon. There is no expression in the metencephalon.

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present	Regionally restricted	6e/f	Expression is present in the ventral, but not dorsal, midbrain.

Structure	Level	Pattern	Image	Note
TS17: fronto-nasal process ectoderm	Present	Regionally restricted	6g/h	Expression is located toward the tips of the frontal-nasal processes. It is in the ectoderm surrounding this region but not in more distal ectoderm.
TS17: fronto-nasal process mesenchyme	Present	Regionally restricted	6g/h	Expression is located toward the tips of the frontal-nasal processes. It is in the neural crest-derived mesenchyme.
TS17: telencephalon	Absent		6g/h

Structure	Level	Pattern	Image	Note
TS15: fronto-nasal process ectoderm	Present		6i/j	Expression is present in the facial epithelium.
TS15: 1st branchial arch mesenchyme derived from neural crest	Present	Regionally restricted	6i/j	Expression present in lateral, but not medial, neural-crest mesenchyme.
TS15: 2nd branchial arch mesenchyme derived from neural crest	Present	Regionally restricted	6i/j	Expression present in lateral, but not medial, neural-crest mesenchyme.
TS15: surface ectoderm	Present	Regionally restricted	6i/j	Expression is present in the ventral region of the spinal cord, excluding the extreme ventral floor plate.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch mesenchyme derived from neural crest	Present	Regionally restricted		Expression present in lateral, but not medial, neural-crest mesenchyme.
TS17: 2nd branchial arch mesenchyme derived from neural crest	Present	Regionally restricted		Expression present in lateral, but not medial, neural-crest mesenchyme.

Structure	Level	Pattern	Image	Note
TS15: trunk mesenchyme	Present	Regionally restricted	6k/l	Expression is present in the lateral mesoderm along the body axis.
TS15: neural tube	Present	Regionally restricted	6k/l	Expression is present in the ventral neural plate.
TS15: neural tube floor plate	Absent		6k/l

Structure	Level	Pattern	Image	Note
TS15: forelimb bud ectoderm	Present	Regionally restricted	7a/b	Expression is restricted to the ventral ectoderm.

Structure	Level	Pattern	Image	Note
TS16: forelimb bud apical ectodermal ridge	Present		7c/d	The highest levels of expression are present in the distal apical ectoderm.
TS16: forelimb bud mesenchyme	Present	Graded	7c/d	Expression is present in the mesenchyme underlying the distal apical ectoderm but only very low levels are seen in the proximal mesenchyme.

Structure	Level	Pattern	Image	Note
TS19: handplate mesenchyme	Present		7e/f	Most expression is distally localized.
TS19: handplate ectoderm	Absent		7e/f

Structure	Level	Pattern	Image	Note
TS20: hand mesenchyme	Present		7g/h	Most expression is distally localized.
TS20: hand epithelium	Absent		7g/h
TS20: forelimb pre-cartilage condensation	Present	Regionally restricted	7g/h	Expression is seen surrounding condensing cartilaginous elements, but no expression is detected in differentiating cartilage or bone.

Structure	Level	Pattern	Image	Note
TS22: hand mesenchyme	Present		7i/j	Most expression is distally localized.
TS22: hand skin	Absent		7i/j
TS22: forelimb cartilage condensation	Present	Regionally restricted	7i/j	Expression is seen surrounding condensing cartilaginous elements, but no expression is detected in differentiating cartilage or bone.

Structure	Level	Pattern	Image	Note
TS22: head	Present	Regionally restricted		Expression is present in the frontal region of the face. It is only present in the mesenchyme, not the overlying ectoderm.