Gene Expression Data
Assay Details
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Assay
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Reference:
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J:14717
Parr BA, et al., Mouse Wnt genes exhibit discrete domains of expression in the early embryonic CNS and limb buds. Development. 1993 Sep;119(1):247-61
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5296979
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Gene symbol:
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Wnt5a
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Gene name:
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wingless-type MMTV integration site family, member 5A
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Probe:
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Wnt5a cDNA1
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Probe preparation:
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Antisense labelled with S35 RNA
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Visualized with:
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Autoradiography
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Results
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Specimen
3:
embryonic day 9.5
(more  )
Specimen
3:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: future forebrain |
Present |
Non-Uniform |
3_5
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Expression is primarily in the ventral region.
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Specimen
Not shown:
embryonic day 9.0
(more )
Note: This embryo is at the 14 somite stage.
Specimen
Not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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Not Specified
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Note:
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This embryo is at the 14 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future brain |
Present |
Regionally restricted |
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Expression is present in the ventral half of the brain.
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Specimen
5A:
embryonic day 9.5
(more )
Specimen
5A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Specimen
5B:
embryonic day 9.5
(more )
Specimen
5B:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Specimen
5C:
embryonic day 9.5
(more )
Specimen
5C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: diencephalon |
Strong |
Regionally restricted |
5. Part 1_5a C
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In the caudal diencephalon, there is strong expression in the ventral half.
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Specimen
5D:
embryonic day 9.5
(more )
Specimen
5D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Specimen
5E:
embryonic day 9.5
(more )
Specimen
5E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Specimen
5F:
embryonic day 9.5
(more )
Specimen
5F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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Specimen
8:
embryonic day 9.5
(more )
Specimen
8:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: forelimb bud mesenchyme |
Present |
Regionally restricted |
8_5a
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Expression is present in a proximal-distal gradient along the limb mesenchyme and ectoderm, with the higest level of expression seen in the distal ectoderm of the future apical ecctodermal ridge and the underlying distal mesenchyme.
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| TS15: forelimb bud ectoderm |
Present |
Regionally restricted |
8_5a
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Expression is present in a proximal-distal gradient along the limb mesenchyme and ectoderm, with the highest level of expression seen in the distal ectoderm of the future apical ecctodermal ridge and the underlying distal mesenchyme.
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| TS15: forelimb bud presumptive apical ectodermal ridge |
Present |
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8_5a
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| TS15: neural tube lateral wall |
Present |
Regionally restricted |
8_5a
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Expression is present in ventral portions of the spinal cord. It is confined to the most rostral part of the spinal cord, though, as it is not found at the level of the forelimbs.
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| TS15: neural tube floor plate |
Absent |
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8_5a
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Analysis Tools