Selenon RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:153128 Castets P, et al., Selenoprotein N is dynamically expressed during mouse development and detected early in muscle precursors. BMC Dev Biol. 2009;9:46
Assay type:	RT-PCR
MGI Accession ID:	MGI:5296935
Gene symbol:	Selenon
Gene name:	selenoprotein N

Probe:	Sepn1-pA, Sepn1-pB
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR. Normalization was relative to the 18s gene.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
E5.5	E5.5	TS7: embryo	Present	0.5 µg; total RNA	C57BL/10		Not Specified
E7.5	E7.5	TS11: embryo	Present	0.5 µg; total RNA	C57BL/10		Not Specified
E9.5	E9.5	TS15: embryo	Present (a)	0.5 µg; total RNA	C57BL/10		Not Specified
E10.5	E10.5	TS17: embryo	Present	0.5 µg; total RNA	C57BL/10		Not Specified
E11.5	E11.5	TS19: embryo	Present	0.5 µg; total RNA	C57BL/10		Not Specified
E12.5	E12.5	TS20: embryo	Present (b)	0.5 µg; total RNA	C57BL/10		Not Specified
E13.5	E13.5	TS21: embryo	Present	0.5 µg; total RNA	C57BL/10		Not Specified
E15.5	E15.5	TS23: embryo	Present	0.5 µg; total RNA	C57BL/10		Not Specified
E18.5	E18.5	TS26: embryo	Present (c)	0.5 µg; total RNA	C57BL/10		Not Specified

Notes:
(a) Expression level was higher at E9.5 than at E5.5 (6-fold increase).
(b) Expression level was higher at E12.5 than at E5.5 (10-fold increase).
(c) Expression level was decreased five-fold from E12.5 to E18.5.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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