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Gene Expression Data
Assay Details
Assay
Reference: J:176514 Kim KH, et al., Gas6 downregulation impaired cytoplasmic maturation and pronuclear formation independent to the MPF activity. PLoS One. 2011;6(8):e23304
Assay type: RT-PCR
MGI Accession ID: MGI:5296807
Gene symbol: Gas6
Gene name: growth arrest specific 6
Probe: Gas6-B-For, Gas6-B-Rev
Probe preparation: labelled with SYBR green
Assay notes: Assay was measured by quantitative real-time PCR. Relative expression levels were calculated from CT values and normalized to added GFP synthetic RNA, and the expression ratio was calculated against expression in the germinal vesicle (GV) oocyte. Experiments were repeated at least three times using different sets of embryos (additional information supplied by authors).
Results
Image: 1B ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 175 bp Amount Genetic BackgroundMutant Allele(s)Sex Note
GV P adult TS28: primary oocyte Strong Not Specified; poly-A+ RNA ICR Female germinal vesicle oocyte
MII P adult TS28: secondary oocyte Strong Not Specified; poly-A+ RNA ICR Female metaphase II oocyte
PN E0.5 (a) TS1: 1-cell stage conceptus Present Not Specified; poly-A+ RNA ICR Not Specified pronulei/one cell stage
2C E1.0 (a) TS2: 2-cell stage conceptus Absent Not Specified; poly-A+ RNA ICR Not Specified two-cell stage
4C E2.0 (a) TS3: 4-cell stage conceptus Absent Not Specified; poly-A+ RNA ICR Not Specified four-cell stage
8C E2.5 (a) TS3: 8-cell stage conceptus Present Not Specified; poly-A+ RNA ICR Not Specified eight-cell stage
MO E3.0 (a) TS3: morula-stage conceptus Present Not Specified; poly-A+ RNA ICR Not Specified morula stage
BL E3.5 (a) TS4: blastocyst-stage conceptus Present Not Specified; poly-A+ RNA ICR Not Specified blastocyst stage
Notes:
(a) Age assigned by curator based on morphological criteria supplied by authors.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
MGI 6.23 		The Jackson Laboratory