Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:151682
Kim JI, et al., Expression of CPI-17 in smooth muscle during embryonic development and in neointimal lesion formation. Histochem Cell Biol. 2009 Aug;132(2):191-8
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:5295974
|
|
Gene symbol:
|
Ppp1r14a
|
|
Gene name:
|
protein phosphatase 1, regulatory inhibitor subunit 14A
|
|
Antibody:
|
Anti-CPI-17
|
|
Detection system:
|
Secondary antibody
|
|
|
Results
|
Specimen
2A:
embryonic day 14.0
(more  )
Note: Double labeled: red - Ppp1r14a; green - Acta2 (assay MGI:5295976).
Specimen
2A:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Ppp1r14a; green - Acta2 (assay MGI:5295976).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: arterial system |
Present |
|
2A Merged
,
2A CPI-17
|
Co-expression with smooth muscle alpha-actin was noted in arterial smooth muscle cells.
|
Specimen
2Ba:
embryonic day 10.0
(more )
Note: Double labeled: red - Ppp1r14a; green - Acta2 (assay MGI:5295976).
Specimen
2Ba:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Ppp1r14a; green - Acta2 (assay MGI:5295976).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS16: arterial system |
Present |
|
2B E10
|
Co-expression with smooth muscle alpha-actin was noted in arterial smooth muscle cells.
|
Specimen
2Bb:
embryonic day 14.0
(more )
Note: Double labeled: red - Ppp1r14a; green - Acta2 (assay MGI:5295976).
Specimen
2Bb:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 14.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Ppp1r14a; green - Acta2 (assay MGI:5295976).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS22: arterial system |
Present |
|
2B E14
|
Co-expression with smooth muscle alpha-actin was noted in arterial smooth muscle cells. The relative expression level appeared to be higher in arterial smooth muscle at E14 compared to E10.
|
Specimen
2Bc:
embryonic day 17.0
(more )
Note: Double labeled: red - Ppp1r14a; green - Acta2 (assay MGI:5295976).
Specimen
2Bc:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 17.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Ppp1r14a; green - Acta2 (assay MGI:5295976).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: arterial system |
Present |
|
2B E17
|
Co-expression with smooth muscle alpha-actin was noted in arterial smooth muscle cells.
|
Specimen
2C E10:
embryonic day 10.0
(more )
Note: Double labeled: red - Ppp1r14a; green - Acta2 (assay MGI:5295976).
Specimen
2C E10:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 10.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Ppp1r14a; green - Acta2 (assay MGI:5295976).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS17: cardiac muscle tissue |
Present |
|
2C CPI-17 E10
,
2C Merged E10
|
Co-expression with smooth muscle alpha-actin was noted in cardiac muscle.
|
Specimen
2C E17:
embryonic day 17.0
(more )
Note: Double labeled: red - Ppp1r14a; green - Acta2 (assay MGI:5295976).
Specimen
2C E17:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 17.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Ppp1r14a; green - Acta2 (assay MGI:5295976).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: cardiac muscle tissue |
Present |
|
2C Merged E17
,
2C CPI-17 E17
|
Co-expression with smooth muscle alpha-actin was noted in cardiac muscle.
|
Specimen
4 Intestine:
embryonic day 17.0
(more )
Note: Double labeled: red - Ppp1r14a; green - Acta2 (assay MGI:5295976).
Specimen
4 Intestine:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 17.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Ppp1r14a; green - Acta2 (assay MGI:5295976).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: intestine muscularis |
Present |
Regionally restricted |
4_CPI-17 Intestine
,
4_Merged Intestine
,
4_Boxed area Intestine
|
Expression was detected equally and prominently in both external and internal layers of smooth muscle in the intestinal wall. Expression overlapped but was not identical with smooth muscle alpha-actin.
|
| TS25: intestine epithelium |
Present |
|
4_Merged Intestine
,
4_CPI-17 Intestine
,
4_Boxed area Intestine
|
Expression in the intestinal epithelium did not overlap with smooth muscle alpha-actin.
|
Specimen
4 Lung:
embryonic day 17.0
(more )
Note: Double labeled: red - Ppp1r14a; green - Acta2 (assay MGI:5295976).
Specimen
4 Lung:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 17.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Ppp1r14a; green - Acta2 (assay MGI:5295976).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: lung |
Present |
Widespread |
4_CPI-17 Lung
,
4_Boxed area Lung
,
4_Merged Lung
|
|
| TS25: lung alveolus |
Present |
Regionally restricted |
4_Merged Lung
,
4_CPI-17 Lung
,
4_Boxed area Lung
|
Expression was detected in epithelial cells of alveoli.
|
Specimen
4 Ganglion:
embryonic day 17.0
(more )
Note: Double labeled: red - Ppp1r14a; green - Acta2 (assay MGI:5295976).
Specimen
4 Ganglion:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 17.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: red - Ppp1r14a; green - Acta2 (assay MGI:5295976).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS25: ganglion |
Present |
Regionally restricted |
4_Boxed area Ganglion
,
4_Merged Ganglion
,
4_CPI-17 Ganglion
|
A punctate staining pattern indicated expression in neuronal cells.
|
| TS25: arterial system |
Present |
|
4_Merged Ganglion
,
4_Boxed area Ganglion
,
4_CPI-17 Ganglion
|
|
Specimen
not shown:
embryonic day 17.0
(more )
Note: Also stained for Acta2 (assay MGI:5295976).
Specimen
not shown:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 17.0
|
|
Age Note:
|
Age of embryo at noon of plug day not specified in reference.
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Also stained for Acta2 (assay MGI:5295976).
|
|
Analysis Tools