Probe:	Dll3 cDNA2
Probe preparation:	Antisense RNA
Assay notes:	It is possible that Probe Dll3 cDNA3 (MGI:2446514) was used for this assay. Embryos were postfixed in 4% paraformaldehyde, 0.1% glutaraldehyde in PBS.

Structure	Level	Pattern	Image	Note
TS9: embryo	Weak	Regionally restricted	5A	Expression only in the epiblast.

Structure	Level	Pattern	Image	Note
TS10: embryo	Weak	Regionally restricted	5B	Expression in the epiblast. Expression in epiblast appears considerably lower than that in the mesoderm.
TS10: embryo mesoderm	Present		5B	Expression in the nascent mesoderm as it emerges from the primitive streak.

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Present	Regionally restricted	5C	Expression extends along its entire length but are absent from the node.
TS10: embryo	Present	Regionally restricted	5C	Expression in epiblast cells adjacent to the primitive streak. Expression in epiblast appears considerably lower than that in the mesoderm.
TS10: embryo mesoderm	Present	Regionally restricted	5C	Expression in the nascent mesoderm as it emerges from the primitive streak.
TS10: node	Absent		5C

Structure	Level	Pattern	Image	Note
TS10: primitive streak	Present		5D
TS10: embryo mesoderm	Present	Regionally restricted	5D	Expression is restricted to the distal/posterior nascent mesoderm, fated to become paraxial mesoderm.
TS10: node	Absent		5D

Structure	Level	Pattern	Image	Note
TS13: primitive streak	Present		6A
TS13: trunk somite	Present	Regionally restricted	6A	Expression in the nascent somites.
TS13: future brain	Weak	Regionally restricted	6A	Expression in the anterior neuroectoderm/presumptive brain region at a low level.
TS13: trunk unsegmented mesenchyme	Present		6A	Expression in the presomitic mesoderm.

Structure	Level	Pattern	Image	Note
TS13: trunk unsegmented mesenchyme	Present		6B
TS13: intermediate mesenchyme	Absent		6B
TS13: lateral plate mesoderm	Absent		6B
TS13: notochord	Absent		6B	No expression in axial mesoderm.

Structure	Level	Pattern	Image	Note
TS13: trunk unsegmented mesenchyme	Strong		6C	Expression is highest in the presomitic mesoderm. Expression in the anterior boundary of the next somite that is to form.
TS13: trunk somite	Present	Regionally restricted	6C	Expression in the anterior boundary of the nascent somite and the somite in the process of of forming or is to form. Expression appears to cease as somite mature.

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Strong	Punctate	6D	Expression in the dorsal region.
TS13: primitive streak	Present		6D
TS13: trunk unsegmented mesenchyme	Present		6D

Structure	Level	Pattern	Image	Note
TS15: facio-acoustic preganglion complex	Present		6E
TS15: glossopharyngeal preganglion	Present		6E
TS15: trigeminal preganglion	Present		6E
TS15: vagus preganglion	Present		6E
TS15: future spinal cord	Present	Punctate	6E	Along the length of the spinal cord.
TS15: nasal process	Present		6E	Expression in the nasal pits.
TS15: peripheral nervous system	Present	Regionally restricted	6E	Expression in the sympathetic chain.
TS15: future hindbrain	Present	Punctate	6E
TS15: future midbrain	Present	Punctate	6E

Structure	Level	Pattern	Image	Note
TS15: dorsal root ganglion	Present		6F
TS15: tail	Present		6F	Expression in the primitive streak/tail bud.

Structure	Level	Pattern	Image	Note
TS8: epiblast	Present			Expression was detected immediately prior to primitive streak formation.