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Gene Expression Data
Assay Details
Assay
Reference: J:10568 Scholer HR, et al., Oct-4: a germline-specific transcription factor mapping to the mouse t-complex. EMBO J. 1990 Jul;9(7):2185-95
Assay type: RNA in situ
MGI Accession ID: MGI:5295173
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Probe: fragment C
Probe preparation: Antisense labelled with S35 RNA
Visualized with: Autoradiography
Assay notes: Probes were subjected to limited alkaline hydrolysis to achieve an optimum probe length of 100-200 nucleotides.
Results
Specimen 3b: embryonic day 3.5 (more )
Note: Early blastocyst.
Structure Level Pattern Image Note
TS4: inner cell mass Present 3b
Specimen 3c: embryonic day 3.5 (more )
Note: Expanding blastocyst.
Structure Level Pattern Image Note
TS4: inner cell mass Present 3c
Specimen 3d: embryonic day 4.0 (more )
Note: Late blastocyst.
Structure Level Pattern Image Note
TS5: inner cell mass Present 3d
Specimen 3e: postnatal (more )
Structure Level Pattern Image Note
TS28: oocyte Present 3e
TS28: oviduct Absent 3e
Specimen 3f: postnatal (more )
Structure Level Pattern Image Note
TS28: oocyte Present 3f
Specimen 3g: postnatal (more )
Structure Level Pattern Image Note
TS28: oocyte Present 3g
Specimen 4a,b: embryonic day 7.0 (more )
Note: Brightfield/darkfield pair.
Structure Level Pattern Image Note
TS10: embryo ectoderm Strong 4a , 4b
TS10: allantois Absent 4b , 4a
TS10: embryo endoderm Absent 4b , 4a
TS10: extraembryonic component Absent 4b , 4a
TS10: trophectoderm Absent 4b , 4a
Specimen 4c: embryonic day 7.0 (more )
Structure Level Pattern Image Note
TS10: embryo ectoderm Present 4c
Specimen 4d: embryonic day 7.5 (more )
Structure Level Pattern Image Note
TS11: embryo ectoderm Strong 4d
TS11: embryo mesoderm Absent 4d
Specimen 5a,b: embryonic day 8.0 (more )
Note: Brightfield/darkfield pair.
Structure Level Pattern Image Note
TS12: neural ectoderm Strong 5b , 5a
Specimen 5c,d: embryonic day 8.5 (more )
Note: 5-6 somites. Brightfield/darkfield pair.
Structure Level Pattern Image Note
TS13: neural tube Present 5d , 5c
TS13: future spinal cord neural fold Present Graded 5c , 5d
Specimen 5f: embryonic day 11.0 (more )
Structure Level Pattern Image Note
TS19: primordial germ cell of gonad Strong 5f
TS19: nervous system Absent 5f
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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12/30/2025
MGI 6.24 		The Jackson Laboratory