Lgr5tm2(Hbegf/EGFP)Fjs
Targeted Allele Detail
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| Symbol: |
Lgr5tm2(Hbegf/EGFP)Fjs |
| Name: |
leucine rich repeat containing G protein coupled receptor 5; targeted mutation 2, Frederick J de Sauvage |
| MGI ID: |
MGI:5294798 |
| Synonyms: |
Lgr5DTR, Lgr5DTR-EGFP, Lgr5-DTR-GFP, Lgr5-EGFP-DTR, Lgr5tm2(DTR/EGFP)Fjs |
| Gene: |
Lgr5 Location: Chr10:115286219-115423685 bp, - strand Genetic Position: Chr10, 63.51 cM
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| Alliance: |
Lgr5tm2(Hbegf/EGFP)Fjs page
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| Germline Transmission: |
Earliest citation of germline transmission:
J:177145
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| Parent Cell Line: |
C2 (Nagy) (ES Cell)
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| Strain of Origin: |
C57BL/6NTac
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| Allele Type: |
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Targeted (Inserted expressed sequence, Null/knockout, Reporter) |
| Mutations: |
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Insertion, Intragenic deletion
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Lgr5tm2(Hbegf/EGFP)Fjs expresses
1 gene
Knock-in expresses:
| Organism |
Expressed Gene |
Note |
| Not Specified |
Hbegf |
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Mutation details: A targeting vector was generated so that a DTR-EGFP-pA-loxP-neo-loxP cassette (diphtheria toxin (DT) cDNA linked in-frame to EGFP polyA-floxed neo) was inserted immediately after the ATG codon in exon 1 of the Lgr5 locus (deleting the remainder of exon 1 and splice donor site in intron 1 - a 212 bp deletion) by homologous recombination in C2 ES cells. Correctly targeted clones were transfected with an cre plasmid to remove the neo cassette.
(J:177145)
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View phenotypes and curated references for all genotypes (concatenated display).
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Carrying any Lgr5 Mutation: |
57 strains or lines available
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| Original: |
J:177145 Tian H, et al., A reserve stem cell population in small intestine renders Lgr5-positive cells dispensable. Nature. 2011 Oct 13;478(7368):255-9 |
| All: |
47 reference(s) |
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Analysis Tools
