Probe:	Mcpt6 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS22: skin	Present	Regionally restricted	2E	Expression was detected in the skin covering the neck region mostly confined to the regio subhyoidea and regio trachealis.

Structure	Level	Pattern	Image	Note
TS22: skin	Present	Regionally restricted	2H	Expression was detected in the back skin correlating to the regio vertebralis thoracis and regio lumbalis.

Structure	Level	Pattern	Image	Note
TS22: skin	Present	Regionally restricted	2K	Expression was detected in the skin around the genital tubercle and also at the tip of the tail corresponding to the regio apicis caudae.

Structure	Level	Pattern	Image	Note
TS24: skin	Present			The identical expression profile to E14.5 was detected at E16.5 in a slightly pronounced way.

Structure	Level	Pattern	Image	Note
TS26: skin	Present	Regionally restricted	3K	Expression was detected in the skin adjacent to the spinal cord.
TS26: lumbar vertebra	Present	Regionally restricted	3K	Expression was detected in the ossification within the cartilage primordium of the lumbar vertebral body.
TS26: spinal cord lateral wall	Absent		3K	No signal was detected in the wall of the spinal cord of the mid lumbar region.
TS26: lumbar intervertebral disc	Absent		3K	No signal was detected in the central part of the intervertebral disc.

Structure	Level	Pattern	Image	Note
TS26: tongue	Strong	Regionally restricted	3L	Expression was detected in the intrinsic muscles of the tongue.
TS26: lower lip	Strong	Regionally restricted	3L	Expression was detected in teh regio mandibularis comprising the lower lip and the area around the genioglossal muscle in the lower lip.

Structure	Level	Pattern	Image	Note
TS26: prepuce of male	Present		3M
TS26: scrotal fold skin	Present	Regionally restricted	3M	Expression was localized to the skin of the scrotum.

Structure	Level	Pattern	Image	Note
TS26: tail	Strong	Regionally restricted	3N	Expression was detected in the tail tip.
TS26: skin	Strong	Regionally restricted	3N	Expression was detected in the skin around the anal pit.

Structure	Level	Pattern	Image	Note
TS26: skin	Present		4A merge , 4A mcpt6	Double in situ hybridization indicated a 100% overlap of Cma1 (mcpt5) and Tpsb2 (mcpt6).

Structure	Level	Pattern	Image	Note
TS26: dermis	Strong	Regionally restricted		Expression was restricted to the papillary and reticular layer of the dermis.