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Gene Expression Data
Assay Details
Assay
Reference: J:174497 Funatsu N, et al., Gene expression analysis of the late embryonic mouse cerebral cortex using DNA microarray: identification of several region- and layer-specific genes. Cereb Cortex. 2004 Sep;14(9):1031-44
Assay type: RT-PCR
MGI Accession ID: MGI:5293693
Gene symbol: Ngef
Gene name: neuronal guanine nucleotide exchange factor
Probe: Ngef-pA, Ngef-pB
Probe preparation: labelled with SYBR green
Assay notes: Quantitative RT-PCR. The comparative cycle threshold value (CT) of each dissected hemisphere region was compared to that of E16.5 hemispheres using the GAPDH gene as an endogenous control. The resulting values were defined as ratio value and presented in a bar graph.
Results Image: 3M QRT-PCR
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
M E16.5 TS24: telencephalon Present 1 µg; poly-A+ RNA ICR Not Specified Medial region.
D E16.5 TS24: telencephalon Present 1 µg; poly-A+ RNA ICR Not Specified Dorsal region.
L E16.5 TS24: telencephalon Present (a) 1 µg; poly-A+ RNA ICR Not Specified Lateral region.
R E16.5 TS24: telencephalon Present 1 µg; poly-A+ RNA ICR Not Specified Rostral region.
O E16.5 TS24: telencephalon Present 1 µg; poly-A+ RNA ICR Not Specified Occipital region.
Notes:
(a) The lateral region was significantly the highest among the five regions.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/09/2024
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