Probe:	Mmp2 probe2
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography

Structure	Level	Pattern	Image	Note
TS21: jaw mesenchyme	Present	Uniform	1a , 1d	Evenly distributed expression in mesenchyme, including mesenchyme around the tooth bud.
TS21: tooth epithelium	Absent		1d , 1a

Structure	Level	Pattern	Image	Note
TS22: dental papilla	Present	Regionally restricted	1b , 1e	Slightly enhanced expression in dental papilla mesenchyme as compared to rest of mesenchyme.
TS22: tooth epithelium	Absent		1e , 1b
TS22: lower jaw mesenchyme	Present	Regionally restricted	1e , 1b	Elevated expression in osteoblastic cells forming mandibular bone.
TS22: mandible	Present	Regionally restricted	1e , 1b	Elevated expression in osteoblastic cells forming mandibular bone.
TS22: Meckel's cartilage	Absent		1e , 1b

Structure	Level	Pattern	Image	Note
TS23: dental papilla	Present	Uniform	1f , 1c	Even expression in dental papilla mesenchyme of early bell stage tooth.
TS23: lower jaw mesenchyme	Present	Regionally restricted	1c , 1f	Even distribution of expression in dental papilla and dental sac, as well as mesenchyme surrounding the tooth.
TS23: tooth epithelium	Absent		1c , 1f	No expression in epithelium of tooth bud, including epithelially derived stellate reticulum cells.
TS23: stellate reticulum	Absent		1f , 1c

Structure	Level	Pattern	Image	Note
TS28: lower jaw	Present	Regionally restricted	1h , 1g	Strong expression in perichondrium, osteoblasts depositing alveolar bone, and in the bone of the angular process of jaw. No expression in cartilage.

Structure	Level	Pattern	Image	Note
TS26: dental papilla	Present	Regionally restricted	2a , 2d	Expression starts to increase in cuspal tips of bell-stage tooth. Increased expression at onset of odontoblast differentiation in preodontoblasts aligning beneath basement membrane in some of cuspal tips.

Structure	Level	Pattern	Image	Note
TS26: dental papilla	Present	Regionally restricted	2e , 2b	Expression begins to be concentrated in differentiated odontoblasts. Increased expression when odontoblasts are terminally differentiated and start to secrete predentine.

Structure	Level	Pattern	Image	Note
TS27: dental papilla	Strong	Regionally restricted	2f , 2c	Very strong expression in odontoblasts. Expression begins to decrease in tips of cusps where mineralization has started.

Structure	Level	Pattern	Image	Note
TS28: tooth	Present	Regionally restricted	2h , 2g	Slightly stronger expression in dental mesenchyme than in background. No expression in odontoblasts in the most advanced region.

Structure	Level	Pattern	Image	Note
TS26: dental papilla	Present	Regionally restricted	3a , 3d	Expression is evenly distributed in all dental mesenchymal cells. Increased expression at onset of odontoblast differentiation in preodontoblasts aligning beneath basement membrane in some of cuspal tips.

Structure	Level	Pattern	Image	Note
TS27: dental papilla	Present	Regionally restricted	3b , 3e	Stronger expression in the odontoblast layer than elsewhere in dental mesenchyme. Clear gradient of expression from well-differeentiated to less differentiated cells down the cuspal slope.

Structure	Level	Pattern	Image	Note
TS27: dental pulp	Weak	Regionally restricted	3c , 3f	Weak expression in dental pulp mesenchyme.
TS27: dental papilla	Present	Regionally restricted	3f , 3c	Expression in odontoblasts is not elevated as compared to rest of dental mesenchyme. Strong expression in odontoblasts in the less differentiated cervical area. Intense expression in odontoblasts of the 2nd molar.

Structure	Level	Pattern	Image	Note
TS27: dental papilla	Strong	Regionally restricted	4c , 4a	Strong expression in odontoblasts

Structure	Level	Pattern	Image	Note
TS27: molar	Present	Regionally restricted	4b , 4d	No expression in odontoblasts