Antibody:	anti-F4/80
Detection system:	Secondary antibody coupled to horseradish peroxidase
Assay notes:	Fixative was either 10% formaldehyde or 10% formaldehyde mixed with 50% benzoquinone and 5% acetic acid.

Structure	Level	Pattern	Image	Note
TS15: yolk sac	Absent		1A

Structure	Level	Pattern	Image	Note
TS17: yolk sac	Present	Single cells	1C	Expression is in macrophages beneath the blood islands.

Structure	Level	Pattern	Image	Note
TS21: yolk sac visceral endoderm	Present	Regionally restricted	1E	Expression is on the vessel wall on macrophages.

Structure	Level	Pattern	Image	Note
TS25: bone marrow	Present	Single cells	2A	Expression is in macrophages.

Structure	Level	Pattern	Image	Note
TS27: bone marrow	Present		2C	Expression is present in macrophages.

Structure	Level	Pattern	Image	Note
TS28: bone marrow	Present		2E	Expression is present in macrophages.

Structure	Level	Pattern	Image	Note
TS19: liver parenchyma	Present	Single cells	3A	Expression is in macrophages scattered throughout the parenchyma.
TS19: liver sinusoid	Present	Single cells	3A	Macrophages in the lumen of sinusoids.

Structure	Level	Pattern	Image	Note
TS21: liver	Present	Regionally restricted	3C	Positive cells have long extended plasma membrane processes and are associated with hematopoietic clusters.

Structure	Level	Pattern	Image	Note
TS25: liver	Present	Regionally restricted	3E	Positive cells have long extended plasma membrane processes and are associated with hematopoietic clusters.

Structure	Level	Pattern	Image	Note
TS27: liver	Present	Regionally restricted	3G	Positive cells have long extended plasma membrane processes and are associated with hematopoietic clusters.

Structure	Level	Pattern	Image	Note
TS28: liver	Present	Single cells	3I	Expression is on Kupffer cells.

Structure	Level	Pattern	Image	Note
TS28: liver	Present	Single cells	3K	Expression is on Kupffer cells.

Structure	Level	Pattern	Image	Note
TS27: lymph node medulla	Present	Regionally restricted	5A	Macrophages in the lymph node in the medullary sinusoidal space adjacent to the paracortical areas.
TS27: lymph node cortex	Absent		5A

Structure	Level	Pattern	Image	Note
TS28: lymph node medulla	Present	Regionally restricted	5C	Macrophages in the lymph node in the medullary sinusoidal space adjacent to the paracortical areas.
TS28: lymph node cortex	Absent		5C

Structure	Level	Pattern	Image	Note
TS28: lymph node medulla	Present	Regionally restricted	5E	Macrophages in the lymph node in the medullary sinusoidal space adjacent to the paracortical areas.
TS28: lymph node cortex	Absent		5E

Structure	Level	Pattern	Image	Note
TS23: intestine	Present	Single cells	6A	Expression is on macrophages.

Structure	Level	Pattern	Image	Note
TS27: small intestine lamina propria	Present		6C

Structure	Level	Pattern	Image	Note
TS28: intestinal villus	Present		6E

Structure	Level	Pattern	Image	Note
TS22: spleen primordium	Present		7A

Structure	Level	Pattern	Image	Note
TS28: spleen red pulp	Present		7C

Structure	Level	Pattern	Image	Note
TS28: spleen red pulp	Present		7E
TS28: spleen white pulp	Trace		7E

Structure	Level	Pattern	Image	Note
TS28: spleen red pulp	Present		7G	Expression is present in macrophages.
TS28: spleen white pulp	Trace		7G

Structure	Level	Pattern	Image	Note
TS21: thymus primordium	Present	Regionally restricted	8A	Expression is in flattened cells beneath the mesenchyme and in scattered cells in the epithelium.

Structure	Level	Pattern	Image	Note
TS23: thymus primordium	Present	Regionally restricted	8C	Expression is beneath the capsule in flattened cells and in scattered cells in the epithelium.

Structure	Level	Pattern	Image	Note
TS28: thymus cortex	Present		8E
TS28: thymus medullary core	Present		8E

Structure	Level	Pattern	Image	Note
TS22: lung	Present	Single cells	9A	Positive cells are scattered throughout the mesenchyme on interstitial macrophages, absent on endothelial cells.

Structure	Level	Pattern	Image	Note
TS26: lung alveolus	Present	Single cells	9C	Expression is in alveolar macrophages absent on endothelial cells.

Structure	Level	Pattern	Image	Note
TS27: lung alveolus	Present		9E	Expression is in alveolar macrophages absent on endothelial cells.

Structure	Level	Pattern	Image	Note
TS28: lung alveolus	Present		9G	Expression is in alveolar macrophages absent on endothelial cells.

Structure	Level	Pattern	Image	Note
TS21: dermis	Present	Single cells	10A	Expression is on macrophages.
TS21: epidermis	Present	Single cells	10A	Expression is on Langerhans cells.

Structure	Level	Pattern	Image	Note
TS24: dermis	Present	Single cells	10C	Expression is on macrophages.
TS24: epidermis	Present	Single cells	10C	Expression is on Langerhans cells.

Structure	Level	Pattern	Image	Note
TS28: dermis	Present	Single cells	10E	Expression is on macrophages.
TS28: epidermis	Present	Single cells	10E	Expression is on Langerhans cells.