Gene Expression Data
Assay Details
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Assay
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Reference:
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J:97487
Xu J, et al., Normal Lung Development in RAIG1-Deficient Mice Despite Unique Lung Epithelium-Specific Expression. Am J Respir Cell Mol Biol. 2005 May;32(5):381-7
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5290073
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Gene symbol:
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Gprc5a
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Gene name:
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G protein-coupled receptor, family C, group 5, member A
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Reporter:
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lacZ
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Direct Detection
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Assay notes:
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The fixative was 1% paraformaldehyde.
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Results
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Specimen
3A:
embryonic day 14.5; Gprc5atm1Jsx/Gprc5a +
(more  )
Note: Genotype of this specimen was not specified. It was assumed to be heterozygous.
Specimen
3A:
(close  )
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Genetic Background:
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involves: 129X1/SvJ
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Mutant Allele(s):
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Gprc5atm1Jsx/Gprc5a+
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Age:
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embryonic day 14.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Genotype of this specimen was not specified. It was assumed to be heterozygous.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lung |
Absent |
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3A
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Specimen
3B:
embryonic day 16.5; Gprc5atm1Jsx/Gprc5a +
(more )
Note: Genotype of this specimen was not specified. It was assumed to be heterozygous.
Specimen
3B:
(close )
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Genetic Background:
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involves: 129X1/SvJ
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Mutant Allele(s):
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Gprc5atm1Jsx/Gprc5a+
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Age:
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embryonic day 16.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Genotype of this specimen was not specified. It was assumed to be heterozygous.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: lung epithelium |
Present |
Widespread |
3B
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Expression was detected in the epithelium but was broadly detected in both terminal buds and future conducting airway epithelium.
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Specimen
3C:
embryonic day 18.5; Gprc5atm1Jsx/Gprc5a +
(more )
Note: Genotype of this specimen was not specified. It was assumed to be heterozygous.
Specimen
3C:
(close )
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Genetic Background:
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involves: 129X1/SvJ
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Mutant Allele(s):
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Gprc5atm1Jsx/Gprc5a+
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Genotype of this specimen was not specified. It was assumed to be heterozygous.
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Specimen
3D:
embryonic day 18.5; Gprc5atm1Jsx/Gprc5a +
(more )
Note: Genotype of this specimen was not specified. It was assumed to be heterozygous.
Specimen
3D:
(close )
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Genetic Background:
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involves: 129X1/SvJ
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Mutant Allele(s):
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Gprc5atm1Jsx/Gprc5a+
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Genotype of this specimen was not specified. It was assumed to be heterozygous.
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Specimen
3E +/-:
postnatal day 10; Gprc5atm1Jsx/Gprc5a +
(more )
Specimen
3E +/-:
(close )
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Genetic Background:
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involves: 129X1/SvJ
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Mutant Allele(s):
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Gprc5atm1Jsx/Gprc5a+
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Age:
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postnatal day 10
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Specimen
3F:
postnatal adult; Gprc5atm1Jsx/Gprc5a +
(more )
Note: Genotype of this specimen was not specified. It was assumed to be heterozygous.
Specimen
3F:
(close )
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Genetic Background:
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involves: 129X1/SvJ
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Mutant Allele(s):
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Gprc5atm1Jsx/Gprc5a+
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Age:
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postnatal adult
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Genotype of this specimen was not specified. It was assumed to be heterozygous.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: lung |
Present |
Regionally restricted |
3F
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Expression was detected in type I and II pneumocytes in the adult. The only lacZ-positive airway cells were at the junction of bronchioles and alveolar ducts.
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| TS28: lung vascular element |
Absent |
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3F
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Analysis Tools