Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(tetO-Gnai2*,-lacZ)382Kndl
Name:	transgene insertion 382, Eric R Kandel
MGI ID:	MGI:5289796
Synonyms:	Tg(tetO-Gnai2*,-lacZ)1Kndl
Transgene:	Tg(tetO-Gnai2*,-lacZ)382Kndl  Location: unknown
Alliance:	Tg(tetO-Gnai2*,-lacZ)382Kndl page

Strain of Origin:

FVB/NJ

Transgene Type:    Transgenic (Constitutively active, Inducible, Inserted expressed sequence, Reporter) Inducer:    doxycycline/tetracycline Mutation:    Insertion   Tg(tetO-Gnai2*,-lacZ)382Kndl expresses 1 gene Transgene expresses: Organism Expressed Gene Note mouse Gnai2 (MGI:95772)   Mutation details: A cysteine to arginine amino acid substitution at residue 179 (R179C) of the Gnai2 sequence interferes with the ability of the mutant protein to hydrolyze GTP, rendering it constitutively active. This mutant form of the gene was placed under the control of the tetO promoter in these transgenic mice. A second construct expressing bacterial beta-galactosidase (lacZ) under the regulation of a tetO promoter was coinjected with the Gnai2 vector. (J:109030)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:109030 Nicholls RE, et al., mGluR2 acts through inhibitory Galpha subunits to regulate transmission and long-term plasticity at hippocampal mossy fiber-CA3 synapses. Proc Natl Acad Sci U S A. 2006 Apr 18;103(16):6380-5
All:	1 reference(s)