Pramel25 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:174767 Tang F, et al., Deterministic and stochastic allele specific gene expression in single mouse blastomeres. PLoS One. 2011;6(6):e21208
Assay type:	RT-PCR
MGI Accession ID:	MGI:5289265
Gene symbol:	Pramel25
Gene name:	PRAME like 25

Probe:

Gm13023-assay_4-FP_first allele, RP

Assay notes:

These results were reported in Table S7. They were obtained using TaqMan real time PCR using individual blastomeres from 2-cell embryos. Two different primer sets were used with each sample in order to examine allele specific expression. In the Supplementary Materials and Methods, the authors reported that a gene was detected when the real-time PCR reaction had a Ct value of <32. Thus, we have interpreted Ct values of <=31.5 as present and >=32.5 as absent.

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
2-cell-E1-B1-first allele	E1.5	TS2: 2-cell stage embryo	Present (a)	Not Specified; total RNA	MF1		Not Specified	This reaction used blastomere 1 from embryo 1.
2-cell-E1-B1-second allele	Lane not used in this assay
2-cell-E1-B2-first allele	E1.5	TS2: 2-cell stage embryo	Present (b)	Not Specified; total RNA	MF1		Not Specified	This reaction used blastomere 2 from embryo 1.
2-cell-E1-B2-second allele	Lane not used in this assay
2-cell-E2-B1-first allele	E1.5	TS2: 2-cell stage embryo	Present (c)	Not Specified; total RNA	MF1		Not Specified	This reaction used blastomere 1 from embryo 2.
2-cell-E2-B1-second allele	Lane not used in this assay
2-cell-E2-B2-first allele	E1.5	TS2: 2-cell stage embryo	Present (d)	Not Specified; total RNA	MF1		Not Specified	This reaction used blastomere 2 from embryo 2.
2-cell-E2-B2-second allele	Lane not used in this assay
2-cell-E3-B1-first allele	E1.5	TS2: 2-cell stage embryo	Present (e)	Not Specified; total RNA	MF1		Not Specified	This reaction used blastomere 1 from embryo 3.
2-cell-E3-B1-second allele	Lane not used in this assay
2-cell-E3-B2-first allele	E1.5	TS2: 2-cell stage embryo	Present (f)	Not Specified; total RNA	MF1		Not Specified	This reaction used blastomere 2 from embryo 3.
2-cell-E3-B2-second allele	Lane not used in this assay
2-cell-E4-B1-first allele	E1.5	TS2: 2-cell stage embryo	Present (g)	Not Specified; total RNA	MF1		Not Specified	This reaction used blastomere 1 from embryo 4.
2-cell-E4-B1-second allele	Lane not used in this assay
2-cell-E4-B2-first allele	E1.5	TS2: 2-cell stage embryo	Present (h)	Not Specified; total RNA	MF1		Not Specified	This reaction used blastomere 2 from embryo 4.
2-cell-E4-B2-second allele	Lane not used in this assay

Notes:
(a) The averaged Ct value from 3 technical replicates was 16.67591367.
(b) The averaged Ct value from 3 technical replicates was 16.622366.
(c) The averaged Ct value from 3 technical replicates was 15.42451633.
(d) The averaged Ct value from 3 technical replicates was 15.50246633.
(e) The averaged Ct value from 3 technical replicates was 15.278232.
(f) The averaged Ct value from 3 technical replicates was 15.44298067.
(g) The averaged Ct value from 3 technical replicates was 16.43944767.
(h) The averaged Ct value from 3 technical replicates was 16.75428467.

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