Probe:	Lbh cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA

Structure	Level	Pattern	Image	Note
TS28: heart	Present

Structure	Level	Pattern	Image	Note
TS11: mesendoderm	Present	Regionally restricted	3A	Expression was detected in the anterior mesendoderm.
TS11: embryo mesoderm	Present	Regionally restricted	3A	Expression was detected in cells of the presumptive cardiac mesoderm.
TS11: embryo endoderm	Present	Regionally restricted	3A	Expression was detected in the primitive gut endoderm.

Structure	Level	Pattern	Image	Note
TS12: heart	Present	Regionally restricted	3B	Expression was detected in the bilaterally symmetrical crescent of the heart primordia.
TS12: gut	Present	Regionally restricted	3B	Expression was detected in the caudally extending gut tube.

Structure	Level	Pattern	Image	Note
TS14: cardiac muscle tissue	Present		3C
TS14: gut	Present	Regionally restricted	3C	Expression was detected in the gut endothelium.
TS14: branchial arch	Present		3C
TS14: tail bud	Present	Regionally restricted	3C	Expression was detected in the ventral ectodermal ridge.
TS14: sinus venosus	Strong		3C

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Present		3D
TS15: cardiac muscle tissue	Present		3D
TS15: gut	Present	Regionally restricted	3D	Expression was detected in the gut endothelium.
TS15: forelimb bud ectoderm	Strong	Regionally restricted	3D	Expression was detected in the ventral ectoderm of the forelimb.
TS15: sinus venosus	Strong		3D

Structure	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	Present		3E
TS17: gut	Present	Regionally restricted	3E	Expression was detected in the gut endothelium.
TS17: forelimb bud ectoderm	Present	Regionally restricted	3E	Expression was detected in the ventral ectoderm of the forelimbs and hindlimbs.
TS17: hindlimb bud ectoderm	Present	Regionally restricted	3E	Expression was detected in the ventral ectoderm of the forelimbs and hindlimbs.

Structure	Level	Pattern	Image	Note
TS14: foregut epithelium	Present		3F

Structure	Level	Pattern	Image	Note
TS14: tail bud	Present	Regionally restricted	3G	Expression was detected in the ventral ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Present		3H
TS15: oral region	Present	Regionally restricted	3H	Expression was detected in the oral epithelium.

Structure	Level	Pattern	Image	Note
TS17: dorsal root ganglion	Present	Regionally restricted	3I	Expression was detected in neural crest-derived sensory neurons of the dorsal root ganglia.

Structure	Level	Pattern	Image	Note
TS17: trigeminal ganglion	Present		3J

Structure	Level	Pattern	Image	Note
TS11: cardiogenic plate	Weak	Non-Uniform	4A	Expression was stronger in the anterior myocardium.

Structure	Level	Pattern	Image	Note
TS12: cardiogenic plate	Present	Non-Uniform	4E	Expression was stronger in the anterior myocardium.

Structure	Level	Pattern	Image	Note
TS12: cardiogenic plate	Present		4I	Expression levels gradually increased.

Structure	Level	Pattern	Image	Note
TS12: primitive heart tube	Present		4M	Lbh appeared to be more evenly expressed in the linear heart tube.

Structure	Level	Pattern	Image	Note
TS13: sinus venosus	Strong		5A
TS13: endocardial tube	Present		5A	Expression was throughout the heart tube.
TS13: common atrial chamber	Absent		5A

Structure	Level	Pattern	Image	Note
TS14: sinus venosus	Strong		5B
TS14: endocardial tube	Present		5B	Expression was throughout the heart tube.

Structure	Level	Pattern	Image	Note
TS15: sinus venosus	Strong		5C
TS15: outflow tract	Present	Regionally restricted	5C	Expression was detected in the outer curvature of the outflow tract.
TS15: bulbus cordis	Present	Regionally restricted	5C	Expression was detected in the right ventricle. Transcripts localized to the outer proliferative compact layer of the ventricular myocardium and to a much lesser extent in the trabeculae.
TS15: primitive ventricle	Weak		5C	Expression was detected at lower levels in the left ventricle.

Structure	Level	Pattern	Image	Note
TS15: sinus venosus	Strong		5D
TS15: atrioventricular canal	Present		5D
TS15: bulbus cordis	Present	Regionally restricted	5D	Expression was detected in the right ventricle.
TS15: outflow tract	Present	Regionally restricted	5D	Expression was detected in the outer curvature of the outflow tract.
TS15: primitive ventricle	Weak	Regionally restricted	5D	Expression was detected at lower levels in the left ventricle.
TS15: heart atrium	Absent		5D

Structure	Level	Pattern	Image	Note
TS17: bulbus cordis	Present	Regionally restricted	5E	Expression was detected in the right ventricle and the forming ventricular septum.
TS17: atrioventricular canal	Present		5E
TS17: outflow tract	Weak		5E

Structure	Level	Pattern	Image	Note
TS12: cardiogenic plate	Present	Regionally restricted	5F	Bilateral expression was detected in the promyocardium.

Structure	Level	Pattern	Image	Note
TS14: bulbus cordis	Present	Regionally restricted	5G	Expression was detected in the right ventricle.
TS14: outflow tract	Present	Regionally restricted	5G	Expression was detected in the anterior myocardium of the outflow tract.
TS14: primitive ventricle	Weak	Regionally restricted	5G	Reduced levels of expression were noted in the left ventricle.

Structure	Level	Pattern	Image	Note
TS17: bulbus cordis	Present	Regionally restricted	5H	Expression was detected in the right ventricle and the forming ventricular septum. Transcripts localized to the outer proliferative compact layer of the ventricular myocardium and to a much lesser extent in the trabeculae.
TS17: outflow tract	Present	Regionally restricted	5H	Expression was detected in the anterior myocardium of the outflow tract.
TS17: primitive ventricle	Weak	Regionally restricted	5H	Reduced expression was noted in the right ventricle.
TS17: heart atrium	Absent		5H
TS17: atrioventricular canal	Present		5H

Structure	Level	Pattern	Image	Note
TS20: atrioventricular endocardial cushion	Absent		5I
TS20: heart atrium	Absent		5I
TS20: ventricle cardiac muscle	Present	Regionally restricted	5I	Lbh expression in the ventricular myocardium exhibited a temporary sidedness, which was lost by E12.5. Transcripts localized to the outer proliferative compact layer of the ventricular myocardium and to a much lesser extent in the trabeculae.
TS20: interventricular septum	Present		5I

Structure	Level	Pattern	Image	Note
TS14: forelimb bud ectoderm	Present	Regionally restricted	6A	Expression was first detected in the ectoderm of the presumptive forelimb field on the lateral body wall.

Structure	Level	Pattern	Image	Note
TS16: hindlimb bud	Present		6E

Structure	Level	Pattern	Image	Note
TS17: forelimb bud ectoderm	Present	Regionally restricted	7A	A minor domain of expression was detected in the proximal posterior-dorsal nonridge ectoderm.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud apical ectodermal ridge	Present	Widespread	7B	Expression extended throughout the entire apical ectodermal ridge.
TS17: forelimb bud ectoderm	Present	Regionally restricted	7B	The proximal limit of ventral expression was more distal than the proximal extreme of the En1 expression domain (see assay MGI:5289698). There was no expression in the most distal dorsal ectoderm.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud apical ectodermal ridge	Strong		7I	Expression appeared normal in the mutant. Expression was strong in the ventrally expanded apical ectoderm ridge and ectopic apical ectodermal ridge on the ventral side of the mutant limb.