Antibody:	srPLP/srDM20 antibody
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS20: alar columns	Very strong		2A	Expression is in the cell bodies within each layer of the developing spinal cord.
TS20: basal columns	Very strong		2A	Expression is in the cell bodies within each layer of the developing spinal cord.

Structure	Level	Pattern	Image	Note
TS23: ventral grey horn	Strong		2B	Some populations of large motor neurons in the medial and lateral motor columns were intensely labeled.

Structure	Level	Pattern	Image	Note
TS22: basal columns	Strong		2C	Some populations of large motor neurons in the medial and lateral motor columns were intensely labeled.

Structure	Level	Pattern	Image	Note
TS24: ventral grey horn	Strong		2D	Some populations of large motor neurons in the medial and lateral motor columns were intensely labeled.

Structure	Level	Pattern	Image	Note
TS21: facial nerve	Present		3A

Structure	Level	Pattern	Image	Note
TS22: hypoglossal nerve	Present		3B

Structure	Level	Pattern	Image	Note
TS21: trigeminal ganglion	Present		3C	Expression is in sensory pseudounipolar neurons.

Structure	Level	Pattern	Image	Note
TS21: inferior glossopharyngeal ganglion	Present		3D	Expression is in sensory pseudounipolar neurons.
TS21: superior glossopharyngeal ganglion	Present		3D	Expression is in sensory pseudounipolar neurons.
TS21: trigeminal ganglion	Present		3D	Expression is in sensory pseudounipolar neurons.

Structure	Level	Pattern	Image	Note
TS21: tongue extrinsic pre-muscle mass	Very strong		4A	Expression is in both the intrinsic and extrinsic muscles of the tongue.
TS21: tongue muscle	Very strong		4A	Expression is in both the intrinsic and extrinsic muscles of the tongue.

Structure	Level	Pattern	Image	Note
TS21: vertebral axis musculature	Present		4B
TS21: skeletal muscle	Present	Regionally restricted	4B	Expression is in axial-skeletal muscles including the muscles along the spine such as the semispinalis cervicis, internal intercostals, and the external intercostals.

Structure	Level	Pattern	Image	Note
TS23: external oblique muscle	Present		4C
TS23: internal oblique muscle	Present		4C
TS23: transversus abdominis	Present		4C

Structure	Level	Pattern	Image	Note
TS21: cardiac muscle tissue	Very strong		4D

Structure	Level	Pattern	Image	Note
TS21: forelimb musculature	Present
TS21: hindlimb musculature	Present
TS21: intestine	Weak
TS21: liver	Weak
TS21: lung	Weak
TS21: metanephros	Weak
TS21: stomach	Weak
TS21: thymus primordium	Weak
TS21: organ system	Weak	Regionally restricted		Expression is in brown adipose tissue.
TS21: autonomic nervous system	Weak	Regionally restricted		Expression is in the pre- and postganglionic motor neurons of the autonomic nervous system.
TS21: smooth muscle tissue	Weak	Regionally restricted		In the gut.