Summary | Transgene origin | Transgene description | Phenotypes | Find Mice (IMSR) | References

Symbol:	Dync1h1TgTn(sb-rtTA,Tyr)2489AOve
Name:	dynein cytoplasmic 1 heavy chain 1; transgenic transposon lentiviral insertion 2489A, Paul A Overbeek
MGI ID:	MGI:5287503
Synonyms:	OVE#2489A, OVE2498A
Gene:	Dync1h1  Location: Chr12:110567886-110633379 bp, + strand  Genetic Position: Chr12, 60.7 cM
Alliance:	Dync1h1TgTn(sb-rtTA,Tyr)2489AOve page

Strain of Origin:

FVB/N

Transgene Type:    Transgenic (Reporter, Transactivator) Mutation:    Transposon insertion   Mutation details: A lentiviral transgenic approach was used to generate these mice. The SB-sa-IRES-rtTA-pA-SB-Tyro-WPRE-FUGW lentiposon transgene (LV2223) was designed with a Sleeping Beauty (SB) transposon (containing a slice-acceptor::IRES::rtTA::polyA gene trap), a mouse tyrosinase minigene (Tyro), and a woodchuck hepatitis virus post-transcriptional regulatory element (WPRE) in the FUGW self-inactivating HIV-based lentiviral vector backbone. The SB transposon and Tyro minigene replaced the ubiquitin-c promoter and EGFP sequences originally found in the FUGW lentiviral vector. The 1200 bp SB transposon used in this transgene has an inverted repeat/direct repeat sequence (IR/DR; the SB transposon recognition site), an adenovirus splice acceptor, a stop sequence (3xSTOP), an internal ribosome entry site (IRES; from human X-chromosome-linked inhibitor of apoptosis (XIAP)), a sequence encoding a-n optimized form of reverse tetracycline controlled transactivator (rtTA2S; with stop codon), a human growth hormone polyA sequence, and a second IR/DR sequence. The IR/DR sequences are outward-facing (pointed away from the sa-IRES-rtTA-pA). The Tyro minigene is composed of the mouse Tyr enhancer region (623 bp), promoter region (657 bp), and 1566 bp cDNA sequence (including the stop codon); all in sense orientation relative to the FUGW backbone. There is no polyA site between the Tyro minigene and WPRE sequence. The WPRE sequence functions to enhance the mRNA transcript stability. In line OVE2489A, a single copy of the lentiposon inserted into intron 64 [NCBI37/mm9; 3'-111,898,386(-)] in the sense orientation. (J:175597)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Dync1h1 Mutation:	194 strains or lines available

Original:	J:175597 Overbeek PA, Direct Data Submission for Overbeek Lentiviral Transgenic Lines. MGI Direct Data Submission. 2011;
All:	1 reference(s)