Gene Expression Data
Assay Details
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Assay
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Reference:
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J:142123
Ishimura A, et al., Man1, an inner nuclear membrane protein, regulates left-right axis formation by controlling nodal signaling in a node-independent manner. Dev Dyn. 2008 Dec;237(12):3565-76
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5287254
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Gene symbol:
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Lefty2
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Gene name:
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left-right determination factor 2
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Probe:
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Lefty2 probe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
Table 1 1s wild type:
embryonic day 8.0
(more  )
Note: 1 somite stage.
Specimen
Table 1 1s wild type:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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1 somite stage.
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Specimen
Table 1 1s Man1 mutant:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Lemd3Gt(XST167)Byg/Lemd3Gt(XST167)Byg
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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1 somite stage.
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Specimen
3E:
embryonic day 8.0
(more )
Note: 4 somite stage.
Specimen
3E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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4 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: embryo |
Present |
Regionally restricted |
3E
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The major expression domain is in the left lateral plate mesoderm, and the midline expression is marginal.
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| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
3E
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Expression was detected in the left lateral plate mesoderm.
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Specimen
3F:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Lemd3Gt(XST167)Byg/Lemd3Gt(XST167)Byg
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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4 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
3F
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Expression was augmented on both sides of the lateral plate mesoderm in the mutant. Ectopic expression on the right side was restricted to the anterior region.
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| TS12: neural plate |
Present |
Regionally restricted |
3F
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Expression was augmented on both sides of the prospective floor plate in the mutant.
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Specimen
3G:
embryonic day 8.0
(more )
Note: 4 somite stage. Transverse section of the embryo in Fig. 3E.
Specimen
3G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Note:
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4 somite stage. Transverse section of the embryo in Fig. 3E.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
3G
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Expression was detected in the left lateral plate mesoderm.
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Specimen
3H:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Lemd3Gt(XST167)Byg/Lemd3Gt(XST167)Byg
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section from whole mount
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Note:
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4 somite stage. Transverse section of the embryo in Fig. 3F.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: lateral plate mesoderm |
Present |
Regionally restricted |
3H
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Expression was augmented on both sides of the lateral plate mesoderm in the mutant.
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| TS12: neural plate |
Present |
Regionally restricted |
3H
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Expression was augmented on both sides of the prospective floor plate in the mutant.
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Specimen
3I:
embryonic day 8.5
(more )
Note: 8-10 somite stage.
Specimen
3I:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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8-10 somite stage.
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Specimen
3J:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Lemd3Gt(XST167)Byg/Lemd3Gt(XST167)Byg
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Note:
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8-10 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: lateral plate mesoderm |
Present |
Regionally restricted |
3J
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Bilateral expression was still observed at the 8-10 somite stage in the mutant.
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Analysis Tools