Ptpn6 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:157883 Reinhard J, et al., Protein tyrosine phosphatases expression during development of mouse superior colliculus. Exp Brain Res. 2009 Dec;199(3-4):279-97
Assay type:	RT-PCR
MGI Accession ID:	MGI:5286975
Gene symbol:	Ptpn6
Gene name:	protein tyrosine phosphatase, non-receptor type 6

Probe:	PTP1C primer set1
Probe preparation:	labelled with SYBR green
Assay notes:	This assay is a quantitative RT-PCR. The data was presented in a bar graph representing the relative expression ratio of the gene in relation to 2 housekeeping genes (beta-actin and cyclophilin) and was normalized to the expression level at E13. The primers amplify a 137bp product.

Results

Image: 1A PTP1C

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified (a)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
E13	E13.0	TS21: midbrain	Present	0.02 µg; total RNA	NMRI		Not Specified	developing superior colliculus
E15	E15.0	TS23: superior colliculus	Present	0.02 µg; total RNA	NMRI		Not Specified
E18	E18.0	TS26: superior colliculus	Present	0.02 µg; total RNA	NMRI		Not Specified
P0	P0	TS27: superior colliculus	Present	0.02 µg; total RNA	NMRI		Not Specified
P4	P4	TS28: superior colliculus	Present	0.02 µg; total RNA	NMRI		Not Specified
P12	P12	TS28: superior colliculus	Present	0.02 µg; total RNA	NMRI		Not Specified
P20	P20	TS28: superior colliculus	Present	0.02 µg; total RNA	NMRI		Not Specified

Notes:
(a) Expression level was downregulated slightly at E15 and E18. At P0 the expression level was the same as at E13. From P4 to P20, a slight upregulation of the gene was observed (statistically not significant).

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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