Gene Expression Data
Assay Details
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Assay
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Reference:
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J:31260
Wuenschell CW, et al., Embryonic mouse lung epithelial progenitor cells co-express immunohistochemical markers of diverse mature cell lineages. J Histochem Cytochem. 1996 Feb;44(2):113-23
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5286166
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Gene symbol:
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Scgb1a1
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Gene name:
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secretoglobin, family 1A, member 1
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Antibody:
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anti-CC10
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Detection system:
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Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
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Results
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Specimen
not shown:
embryonic day 13.0
(more  )
Specimen
not shown:
(close  )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: lung epithelium |
Present |
Regionally restricted |
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Expression in epithelium of smaller airways; no expression in epithelium of larger airways.
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Specimen
1B:
embryonic day 14.0
(more )
Specimen
1B:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lung epithelium |
Present |
Regionally restricted |
1B
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Expression in epithelium of smaller, more peripheral airways; expression tended to be continuous, strongest at luminal edge. Little or no expression in epithelium of larger proximal airways.
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Specimen
1F:
embryonic day 14.0
(more )
Specimen
1F:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: lung epithelium |
Present |
Regionally restricted |
1F
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Gradient of expression from tall columnar epithelium of larger airways, with increasing expression in low cuboidal epithelium of smaller airways.
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Specimen
not shown:
embryonic day 15.0
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 15.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: lung epithelium |
Present |
Regionally restricted |
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Expression confined to epithelium of small airways; maximal level of expression not as high as at E14.
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Specimen
2B:
embryonic day 16.0
(more )
Specimen
2B:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 16.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: lung epithelium |
Present |
Regionally restricted |
2B
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Almost no expression in small airway epithelium. Expression in epithelium of all large airways, in the presumptive Clara cell population.
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Specimen
not shown:
embryonic day 17.0
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 17.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS25: lung epithelium |
Present |
Regionally restricted |
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Pattern of expression similar to those described for more mature specimens of E16 lung tissue.
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Specimen
3B:
embryonic day 18.0
(more )
Specimen
3B:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: lung epithelium |
Present |
Regionally restricted |
3B
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Expression in most but not all cells within large airway epithelium and in restricted regions of small airways, ending abruptly at their distal extremities. Non-expressing cells occurred singly or in small clusters among expressing cells.
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Specimen
3D:
embryonic day 18.0
(more )
Specimen
3D:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: lung epithelium |
Present |
Regionally restricted |
3D
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Little or no expression in cells at the core of the neuroepithelial body (NEB), while many surrounding cells are strongly expressing.
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Specimen
4B:
embryonic day 18.0
(more )
Specimen
4B:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 18.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: lung epithelium |
Present |
Regionally restricted |
4B
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No expression in core of tall neuroendocrine cells in large NEB. Expression in cap of presumptive Clara cells covering luminal surface of NEB. Expression in Clara cell population in all large airway epithelium, extending into proximal small airways.
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| TS26: lung |
Present |
Regionally restricted |
4B
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Expression in large masses of noncellular material in luman of large airways.
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| TS26: lung mesenchyme |
Absent |
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4B
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No expression in lung parenchyma.
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