Gene Expression Data
Assay Details
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Assay
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Reference:
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J:58080
Coffinier C, et al., Essential role for the homeoprotein vHNF1/HNF1beta in visceral endoderm differentiation. Development. 1999 Nov;126(21):4785-94
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5286159
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Gene symbol:
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Hnf1b
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Gene name:
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HNF1 homeobox B
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
2A:
embryonic day 7.5; Hnf1btm1Mya/Hnf1b +
(more  )
Specimen
2A:
(close  )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Hnf1btm1Mya/Hnf1b+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2B:
embryonic day 8.0; Hnf1btm1Mya/Hnf1b +
(more )
Specimen
2B:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Hnf1btm1Mya/Hnf1b+
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Age:
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embryonic day 8.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2C:
embryonic day 8.5; Hnf1btm1Mya/Hnf1b +
(more )
Specimen
2C:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Hnf1btm1Mya/Hnf1b+
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2D:
embryonic day 9.5; Hnf1btm1Mya/Hnf1b +
(more )
Specimen
2D:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Hnf1btm1Mya/Hnf1b+
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: central nervous system |
Present |
Regionally restricted |
2D
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Expression is restricted to a sharp stripe at the level of the otic vesicle and to a broader and more posterior domain.
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| TS15: gut epithelium |
Present |
Regionally restricted |
2D
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A sharp anterior boundary is observed at the level of the pancreas and liver primordia. Expression extends posteriorly to the tip of the gut.
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Specimen
Not shown:
embryonic day 10.0; Hnf1btm1Mya/Hnf1b +
(more )
Note: Embedded in methacrylate.
Specimen
Not shown:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Hnf1btm1Mya/Hnf1b+
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Age:
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embryonic day 10.0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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Embedded in methacrylate.
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Specimen
3, +/-:
embryonic day 7.5; Hnf1btm1Mya/Hnf1b +
(more )
Specimen
3, +/-:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Hnf1btm1Mya/Hnf1b+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3, -/-:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Hnf1btm1Mya/Hnf1btm1Mya
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: conceptus |
Present |
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3-/-
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Homozygotes are severely growth retarded and disorganized.
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Specimen
4A:
embryonic day 7.5; Hnf1btm1Mya/Hnf1b +
(more )
Note: Embedded in methacrylate.
Specimen
4A:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Hnf1btm1Mya/Hnf1b+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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Embedded in methacrylate.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: extraembryonic visceral endoderm |
Present |
Regionally restricted |
4A
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Expression is present in the columnar epithelial cells. There is no expression in the squamous epithelial layer surrounding the gastrulating embryo.
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Specimen
4B:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Hnf1btm1Mya/Hnf1btm1Mya
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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Embedded in methacrylate.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: conceptus |
Present |
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4B
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There are expressing cells, but no clear regionalization of the visceral endoderm is observed.
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Specimen
4C:
(close )
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Genetic Background:
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involves: 129S2/SvPas
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Mutant Allele(s):
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Hnf1btm1Mya/Hnf1btm1Mya
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Resin/plastic
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Note:
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Embedded in methacrylate.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: conceptus |
Present |
Regionally restricted |
4C
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There are expressing cells, but this embryo is highly degenerated and disorganized; no clear regionalization of the visceral endoderm is observed.
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Analysis Tools
