Antibody:	mCXCR2 antibody
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS21: esophagus epithelium	Present	Patchy	2D

Structure	Level	Pattern	Image	Note
TS21: spinal cord floor plate	Present		2E

Structure	Level	Pattern	Image	Note
TS21: epidermis	Present		2F	Shown is back skin.

Structure	Level	Pattern	Image	Note
TS21: liver	Present	Single cells	4A

Structure	Level	Pattern	Image	Note
TS20: heart	Present	Regionally restricted	5A	Expression is in the endothelial cells lining the blood vessels of the heart.

Structure	Level	Pattern	Image	Note
TS20: heart	Present	Regionally restricted	5B	Expression is in the endothelial cells lining the blood vessels of the heart.

Structure	Level	Pattern	Image	Note
TS20: dorsal aorta	Strong		5C	Expression is in endothelial cells.

Structure	Level	Pattern	Image	Note
TS19: heart atrium	Strong		6A
TS19: heart ventricle	Strong		6A

Structure	Level	Pattern	Image	Note
TS19: dorsal mesocardium	Strong		6B
TS19: endocardial lining	Weak		6B

Structure	Level	Pattern	Image	Note
TS21: lung epithelium	Present	Patchy	7B

Structure	Level	Pattern	Image	Note
TS21: thyroid gland	Present		7D

Structure	Level	Pattern	Image	Note
TS19: myotome	Present
TS19: pre-cartilage condensation	Present
TS19: dorsal root ganglion	Strong
TS19: neural tube floor plate	Strong
TS19: notochord	Strong
TS19: sympathetic nervous system	Present	Regionally restricted		Expression is in sympathetic neurons.
TS19: trachea	Present	Patchy		Expression is in the endoderm.
TS19: limb	Absent

Structure	Level	Pattern	Image	Note
TS20: floor plate	Strong
TS20: dorsal aorta	Strong	Regionally restricted		Expression is in endothelial cells.
TS20: blood vessel	Strong	Regionally restricted		Expression is in the endothelial cells in the developing brain.
TS20: head mesenchyme	Present
TS20: trachea	Present	Patchy		Expression is in the endoderm.
TS20: forebrain	Present	Regionally restricted		Expression is in the forbrain and is absent in the ventral midline of the forebrain,
TS20: brain	Present	Regionally restricted		Expression is in the meninges.
TS20: hindbrain	Present	Regionally restricted		Expression is in the ventral midline from the midbrain extending toward the posterior spinal cord.
TS20: midbrain	Present	Regionally restricted		Expression is in the ventral midline from the midbrain extending toward the posterior spinal cord.
TS20: spinal cord	Present	Regionally restricted		Expression is in the meninges and in the ventral midline from the midbrain extending toward the posterior spinal cord.
TS20: dorsal mesocardium	Present
TS20: endocardial lining	Present
TS20: heart atrium	Present
TS20: heart ventricle	Present

Structure	Level	Pattern	Image	Note
TS21: medulla oblongata part of 4th ventricle choroid plexus	Very strong
TS21: embryo	Strong	Regionally restricted		Expression is in the notochord.
TS21: diaphragm	Strong
TS21: lateral ventricle choroid plexus	Strong
TS21: metencephalon part of 4th ventricle choroid plexus	Strong
TS21: stomach mesenchyme	Present	Regionally restricted		Expression is in the smooth muscle of the muscularis mucosa.
TS21: peritoneal component	Present	Regionally restricted		Expression is in the inner lining.
TS21: forebrain	Present	Regionally restricted		Expression is in the hippocampus.
TS21: trachea	Present	Patchy		Expression is in the endoderm.
TS21: diencephalon meninges	Present	Regionally restricted		Expression is in the meningeal cells around the pituitary.
TS21: venous system	Present	Regionally restricted		Expression is in the lining of the central veins in the liver.
TS21: dorsal mesocardium	Present
TS21: endocardial tissue	Present
TS21: heart atrium	Present
TS21: heart ventricle	Present
TS21: hypothalamus	Present
TS21: limb cartilage condensation	Present
TS21: limb epithelium	Present
TS21: olfactory epithelium	Present
TS21: pituitary gland	Present
TS21: telencephalon	Present
TS21: thalamus	Present
TS21: vertebral cartilage condensation	Weak	Regionally restricted
TS21: body wall mesenchyme	Weak	Regionally restricted		Expression is in the body wall muscle.
TS21: dorsal root ganglion	Absent
TS21: intestine	Absent
TS21: pancreas	Absent

Structure	Level	Pattern	Image	Note
TS22: dorsal mesocardium	Present
TS22: endocardial tissue	Present
TS22: heart atrium	Present
TS22: heart ventricle	Present
TS22: intervertebral disc	Present
TS22: oral epithelium	Present
TS22: tongue epithelium	Present
TS22: primary palate mesenchyme	Present	Regionally restricted		Expression is in the cartilaginous cells of the palate.
TS22: primary palate	Present	Regionally restricted		Expression is where the tongue and palate join.
TS22: tongue	Present	Regionally restricted		Expression is where the tongue and palate join.