Gene Expression Data
Assay Details
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Assay
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Reference:
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J:60832
Pattyn A, et al., Control of hindbrain motor neuron differentiation by the homeobox gene Phox2b. Development. 2000 Apr;127(7):1349-58
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:5141494
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Gene symbol:
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Phox2b
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Gene name:
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paired-like homeobox 2b
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
2A:
embryonic day 10.5; Phox2btm1Jbr/Phox2b +
(more  )
Specimen
2A:
(close  )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Phox2btm1Jbr/Phox2b+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
2A
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Expression is present in three longitudinal columns on each side, as well as in migrating cells caudal to rhombomere 6 and axon outgrowths in rhombomeres 2 and 4.
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Specimen
2A':
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Phox2btm1Jbr/Phox2btm1Jbr
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
2A'
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In the mutant, there are fewer expressing cells. Staining in the ventral-most column is preserved only in rhombomere 4. No signs of axon outgrowth or lateral or caudal cell migration are visible.
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Specimen
2B:
embryonic day 11.5; Phox2btm1Jbr/Phox2b +
(more )
Specimen
2B:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Phox2btm1Jbr/Phox2b+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindbrain |
Present |
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2B
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Expressing cells are present in rhombomere 4 and are migrating caudally through rhombomeres 5 and 6.
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Specimen
2B':
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Phox2btm1Jbr/Phox2btm1Jbr
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindbrain |
Present |
Regionally restricted |
2B'
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In the mutant, expression is still present in rhombomere 4. However, the caudal migration of the facial precursors does not occur.
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Specimen
4D:
embryonic day 10.5; Phox2btm1Jbr/Phox2b +
(more )
Specimen
4D:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Phox2btm1Jbr/Phox2b+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: rhombomere 4 |
Present |
Regionally restricted |
4D
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Expression is present in the ventral region.
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Specimen
4D':
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Phox2btm1Jbr/Phox2btm1Jbr
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: rhombomere 4 |
Present |
Regionally restricted |
4D'
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Expression is present in the ventral region. However, in the mutant these cells are not differentiating into postmitotic neurons.
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Specimen
7B:
embryonic day 10.5; Phox2btm1Jbr/Phox2b +
(more )
Specimen
7B:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Phox2btm1Jbr/Phox2b+
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Specimen
7B':
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Phox2btm1Jbr/Phox2btm1Jbr
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Analysis Tools
