Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:174146
Heinonen J, et al., Snorc is a novel cartilage specific small membrane proteoglycan expressed in differentiating and articular chondrocytes. Osteoarthritis Cartilage. 2011 Aug;19(8):1026-35
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:5140398
|
|
Gene symbol:
|
Snorc
|
|
Gene name:
|
secondary ossification center associated regulator of chondrocyte maturation
|
|
Antibody:
|
anti-Snorc
|
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor 488
|
|
Assay notes:
|
A mixture of two antibodies (MGI:5140020 and MGI:5140096) was used in immunohistochemical studies.
|
|
|
Results
|
Specimen
6A:
postnatal day 10
(more  )
Specimen
6A:
(close  )
|
Genetic Background:
|
either: C57BL/6 or (involves: C57BL/6 * DBA)
|
|
Age:
|
postnatal day 10
|
|
Sex:
|
Not Specified
|
|
Type:
|
optical section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: epiphyseal plate proliferative zone |
Present |
|
6A
|
Expression in the cytoplasm, but also next to the inner plasma membrane and at the margins of the chondrocyte lacunae in the knee femoral epiphysis.
|
Specimen
6B:
postnatal day 10
(more )
Specimen
6B:
(close )
|
Genetic Background:
|
either: C57BL/6 or (involves: C57BL/6 * DBA)
|
|
Age:
|
postnatal day 10
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: epiphyseal plate hypertrophic zone |
Present |
|
6B
|
Expression in the cytoplasm, but also next to the inner plasma membrane and at the margins of the chondrocyte lacunae in the knee femoral epiphysis.
|
Specimen
6C:
postnatal month 1
(more )
Specimen
6C:
(close )
|
Genetic Background:
|
either: C57BL/6 or (involves: C57BL/6 * DBA)
|
|
Age:
|
postnatal month 1
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: articular cartilage |
Present |
|
6C
|
Expression in the cytoplasm, but also next to the inner plasma membrane and at the margins of the chondrocyte lacunae in the knee femoral epiphysis.
|
Specimen
6D:
postnatal month 6
(more )
Specimen
6D:
(close )
|
Genetic Background:
|
either: C57BL/6 or (involves: C57BL/6 * DBA)
|
|
Age:
|
postnatal month 6
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: epiphyseal plate |
Present |
Regionally restricted |
6D
|
In the chondrocyte clusters of growth plate, expression was seen in the cells proximal to the articular surface, not in the cells closer to the metaphyseal bone in the knee femoral epiphysis.
|
|
Analysis Tools