Gene Expression Data
Assay Details
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Assay
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Reference:
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J:174146
Heinonen J, et al., Snorc is a novel cartilage specific small membrane proteoglycan expressed in differentiating and articular chondrocytes. Osteoarthritis Cartilage. 2011 Aug;19(8):1026-35
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5140395
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Gene symbol:
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Snorc
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Gene name:
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secondary ossification center associated regulator of chondrocyte maturation
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Antibody:
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anti-Snorc
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Detection system:
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Secondary antibody coupled to horseradish peroxidase
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Assay notes:
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A mixture of two antibodies (MGI:5140020 and MGI:5140096) was used in immunohistochemical studies.
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Results
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Specimen
5A left top:
embryonic day 18.5
(more  )
Specimen
5A left top:
(close  )
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Genetic Background:
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either: C57BL/6 or (involves: C57BL/6 * DBA)
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Age:
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embryonic day 18.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: tibia |
Present |
Regionally restricted |
5A left top
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Intracellular staining was observed throughout the differentiating zones, excluding the superficial two to three cell layers of articular surface and the hypertrophic zone in the proximal tibial head.
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Specimen
5A left bottom:
postnatal day 5
(more )
Specimen
5A left bottom:
(close )
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Genetic Background:
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either: C57BL/6 or (involves: C57BL/6 * DBA)
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Age:
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postnatal day 5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: tibia |
Present |
Regionally restricted |
5A right middle
,
5A left bottom
,
5A right top
,
5A right bottom
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Intracellular staining was observed throughout the differentiating zones, excluding the superficial two to three cell layers of articular surface and the hypertrophic zone in the proximal tibial head.
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| TS28: epiphyseal plate proliferative zone |
Strong |
Regionally restricted |
5A left bottom
,
5A right middle
,
5A right bottom
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Highest expressionwas seen in the proliferating and prehypertrophic zones of the developing growth plate in the proximal tibial head.
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| TS28: epiphyseal plate hypertrophic zone |
Absent |
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5A right bottom
,
5A left bottom
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| TS28: articular cartilage |
Present |
Regionally restricted |
5A left bottom
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No expression in the superficial two to three cell layers in the proximal tibial head.
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Specimen
5B Snorc 2 mo top:
postnatal month 2
(more )
Specimen
5B Snorc 2 mo top:
(close )
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Genetic Background:
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either: C57BL/6 or (involves: C57BL/6 * DBA)
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Age:
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postnatal month 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: articular cartilage |
Present |
Regionally restricted |
5B Snorc 2 mo top
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Intracellular protein was detected in the uncalcified articular cartilage, while below the tidemark no intracellular staining was seen in the proximal tibial head.
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Specimen
5B Snorc 2 mo bottom:
postnatal month 2
(more )
Specimen
5B Snorc 2 mo bottom:
(close )
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Genetic Background:
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either: C57BL/6 or (involves: C57BL/6 * DBA)
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Age:
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postnatal month 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: epiphyseal plate proliferative zone |
Present |
Regionally restricted |
5B Snorc 2 mo bottom
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Major staining was observed in the proliferating/prehypertrophic chondrocytes in the proximal tibial head.
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| TS28: long bone metaphysis |
Present |
Regionally restricted |
5B Snorc 2 mo bottom
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Trabecular staining was seen in the cartilaginous islands of primary spongiosa.
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Specimen
5B Snorc 6 mo top:
postnatal month 6
(more )
Specimen
5B Snorc 6 mo top:
(close )
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Genetic Background:
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either: C57BL/6 or (involves: C57BL/6 * DBA)
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Age:
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postnatal month 6
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: articular cartilage |
Present |
Regionally restricted |
5B Snorc 6 mo top
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Intracellular protein was detected in the uncalcified articular cartilage, while below the tidemark no intracellular staining was seen in the proximal tibial head.
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Specimen
5B Snorc 6 mo bottom:
postnatal month 6
(more )
Specimen
5B Snorc 6 mo bottom:
(close )
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Genetic Background:
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either: C57BL/6 or (involves: C57BL/6 * DBA)
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Age:
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postnatal month 6
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: epiphyseal plate proliferative zone |
Present |
Regionally restricted |
5B Snorc 6 mo bottom
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Major staining was observed in the proliferating/prehypertrophic chondrocytes in the proximal tibial head.
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| TS28: long bone metaphysis |
Present |
Regionally restricted |
5B Snorc 6 mo bottom
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Trabecular staining was seen in the cartilaginous islands of primary spongiosa.
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