Summary | Mutation origin | Mutation description | Phenotypes | Find Mice (IMSR) | References

Symbol:	H2-T3tm2.1(CAG-EGFP)Maoh
Name:	histocompatibility 2, T region locus 3; targeted mutation 2.1, Masato Ohtsuka
MGI ID:	MGI:5056530
Gene:	H2-T3  Location: Chr17:36496463-36501043 bp, - strand  Genetic Position: Chr17, 18.99 cM
Alliance:	H2-T3tm2.1(CAG-EGFP)Maoh page

Germline Transmission:	Earliest citation of germline transmission: J:173808
Parent Cell Line:	E14TG2a (ES Cell)
Strain of Origin:	129P2/OlaHsd

Allele Type:    Targeted (Conditional ready, Null/knockout, RMCE-ready, Reporter) Mutations:    Insertion, Intragenic deletion   Mutation details: Recombinase-mediated cassette exchange (RMCE) replaced the expression cassette between the JT15 and lox2272 sites of H2-T3tm1Maoh with (5' to 3') an IRES, lacZ, polyadenylation sequence, CAG promoter, hygro gene, polyadenylation sequence, FRT site, polyadenylation sequence, and CAG driven EGFP. Flp-mediated recombination removes all but the CAG promoter EGFP reporter construct flanked by an FRT site and the lox2272 site. (J:173808)

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any H2-T3 Mutation:	25 strains or lines available

Original:	J:173808 Ohtsuka M, et al., Pronuclear injection-based mouse targeted transgenesis for reproducible and highly efficient transgene expression. Nucleic Acids Res. 2010 Dec 1;38(22):e198
All:	1 reference(s)