Olfm3 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:171532 Sultana A, et al., Olfactomedin 2: expression in the eye and interaction with other olfactomedin domain-containing proteins. Invest Ophthalmol Vis Sci. 2011 Apr;52(5):2584-92
Assay type:	RT-PCR
MGI Accession ID:	MGI:5056153
Gene symbol:	Olfm3
Gene name:	olfactomedin 3

Probe:	Olfm3-pA, Olfm3-pB
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative PCR. The levels of mRNA were calculated relative to their levels in adult tissues for each gene. Normalization was performed using GAPDH mRNA. The primers that we used amplify all forms of Olfm3.

Results

Image: 3B Olfm3

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
13.5E	E13.5 (a)	TS21: head	Present (b)	2 µg; total RNA	Not Specified		Not Specified	head minus eyes
16.5E	E16.5 (a)	TS24: head	Present (b)	2 µg; total RNA	Not Specified		Not Specified	head minus eyes
19.5E	E19.5 (a)	TS26: brain	Present (b)	2 µg; total RNA	Not Specified		Not Specified
P2	P2	TS27: brain	Present (b)	2 µg; total RNA	Not Specified		Not Specified
P6	P6	TS28: brain	Present (b)	2 µg; total RNA	Not Specified		Not Specified
Ad	P adult	TS28: brain	Present (b)	2 µg; total RNA	Not Specified		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) There was a moderate increase in expression in the course of embryonic development and was stable in the postnatal brain.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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