Reporter:	lacZ
	Direct Detection
Assay notes:	Mirsky's fixative was used.

Structure	Level	Pattern	Image	Note
TS10: neural ectoderm	Present	Regionally restricted	1A	Expression in the region above the node.

Structure	Level	Pattern	Image	Note
TS10: neural ectoderm	Present		1B
TS10: extraembryonic component	Present	Regionally restricted	1B	Expression in extraembryonic vascular progenitors of the chorion.

Structure	Level	Pattern	Image	Note
TS10: neural ectoderm	Present		1C

Structure	Level	Pattern	Image	Note
TS12: future brain	Present	Regionally restricted	1D	Expression in two convergent stripes in the neuroepithelium at the level of rhombomere two.
TS12: dorsal aorta	Weak	Regionally restricted	1D	Faint expression in the rostral-most aspect of the dorsal aorta.

Structure	Level	Pattern	Image	Note
TS12: 1st branchial arch mesenchyme derived from neural crest	Present		1E
TS12: neural ectoderm	Present		1E
TS12: dorsal aorta	Present		1E
TS12: outflow tract	Present	Widespread	1E

Structure	Level	Pattern	Image	Note
TS12: dorsal aorta	Present	Regionally restricted	1F	Expression in the dorsal aorta in rostral and future cervical regions.

Structure	Level	Pattern	Image	Note
TS13: dorsal aorta	Present	Regionally restricted	1G	Expression up to the presomitic mesoderm region.
TS13: 1st branchial arch mesenchyme derived from neural crest	Present		1G
TS13: 2nd branchial arch mesenchyme derived from neural crest	Present		1G

Structure	Level	Pattern	Image	Note
TS13: venous system	Present	Regionally restricted	1H	Expression in the intersomitic veins.

Structure	Level	Pattern	Image	Note
TS13: outflow tract	Strong	Regionally restricted	1I	Expression in the heart is restricted to the lining of the outflow track.
TS13: bulbus cordis	Strong		1I
TS13: 1st branchial arch mesenchyme derived from neural crest	Present		1I
TS13: 2nd branchial arch mesenchyme derived from neural crest	Present		1I

Structure	Level	Pattern	Image	Note
TS14: cardiovascular system	Present		2A	Expression in all vascular progenitors, including the aortic sac.

Structure	Level	Pattern	Image	Note
TS14: primary head vein	Present		2B
TS14: anterior cardinal vein	Present	Regionally restricted	2B	Expression in the anterior vein plexus that occupies the head region.
TS14: arterial system	Present	Regionally restricted	2B	Expression in the main arteries in the head.

Structure	Level	Pattern	Image	Note
TS14: 1st arch artery	Present		2C
TS14: 2nd arch artery	Present		2C
TS14: venous system	Present	Regionally restricted	2C	Expression in the first and second branchial arch veins.

Structure	Level	Pattern	Image	Note
TS14: venous system	Present	Regionally restricted	2D	Expression in the forelimb bud and intersomitic veins.

Structure	Level	Pattern	Image	Note
TS14: dorsal aorta	Present	Widespread	2E	Expression is throughout the dorsal aorta, including the recurved portion in the tail.

Structure	Level	Pattern	Image	Note
TS14: outflow tract	Present	Regionally restricted	2F	Expression in the outflow track is restricted to the lining of the canal.

Structure	Level	Pattern	Image	Note
TS14: venous system	Present	Regionally restricted	2G	Expression in the intersomitic veins.
TS14: blood vessel	Present	Regionally restricted	2G	Expression in the perineural vascular plexus.
TS14: trunk dermomyotome	Present	Regionally restricted	2G	Expression is throughout the ventral portion of the dermomyotome, including both rostral and caudal lips, with the highest levels of expression at the dermomyotome-myotome interface at the forelimb level.
TS14: trunk myotome	Absent		2G	No expression in myoblasts.

Structure	Level	Pattern	Image	Note
TS14: dorsal aorta	Present	Regionally restricted	2H	Expression in the proximal and distal (recurved) dorsal aorta in the tail.

Structure	Level	Pattern	Image	Note
TS14: primitive ventricle	Present	Regionally restricted	2I	Expression in the endocardium lining of the trabeculations.
TS14: common atrial chamber	Present	Regionally restricted	2I	Expression in the endocardium lining of the trabeculations.
TS14: atrioventricular canal	Present	Regionally restricted	2I	Expression in the endocardium lining of the atrio-ventricular canal.

Structure	Level	Pattern	Image	Note
TS17: cardiovascular system	Present		3A	Vascular expression is throughout the embryo, including the dorsal aorta, the perineural vascular plexus and the developing enteric vascular system.

Structure	Level	Pattern	Image	Note
TS17: blood vessel	Present	Regionally restricted	3B	Expression in the main and secondary head vessels.

Structure	Level	Pattern	Image	Note
TS17: blood vessel	Present	Regionally restricted	3C	Expression in the arch vessels and orbital eye vasculature.
TS17: 1st branchial arch ectoderm	Present		3C

Structure	Level	Pattern	Image	Note
TS17: lens pit	Present	Regionally restricted	3D	Expression in the ectodermal layer.

Structure	Level	Pattern	Image	Note
TS17: nasal process	Present	Regionally restricted	3E	Expression in the epithelium between the left and right nasal processes.

Structure	Level	Pattern	Image	Note
TS17: outflow tract	Present	Regionally restricted	3F	Expression in the endocardium lining common ventricular chamber, and the lining of the aortic sac and bulbo-truncal canal in the outflow tract.
TS17: bulbus cordis	Present	Regionally restricted	3F	Expression in the endocardium lining the trabeculations.
TS17: primitive ventricle	Present	Regionally restricted	3F	Expression in the common ventricular chamber.

Structure	Level	Pattern	Image	Note
TS17: nephric duct, mesonephric portion	Present	Regionally restricted	3G	Expression in the epithelium of the mesonephric duct.
TS17: myotome	Present	Regionally restricted	3G	Expression in the muscle progenitors in the central myotome.
TS17: dermatome	Absent		3G
TS17: dorsal aorta	Present		3G
TS17: blood vessel	Present	Regionally restricted	3G	Expression in the perineural vascular plexus.

Structure	Level	Pattern	Image	Note
TS17: foregut	Present	Regionally restricted	3H	Expression in the epithelium.

Structure	Level	Pattern	Image	Note
TS17: blood vessel	Present	Regionally restricted	3I	Expression in the enteric vascular system.
TS17: hindgut epithelium	Absent		3I

Structure	Level	Pattern	Image	Note
TS19: cardiovascular system	Present		4A	There is a general downregulation of the vascular expression, although present in most of the head vessels. Expression is downregulated in all chambers and in the lining of the bulbo-ventricular canal, while being upregulated in the endocardial cushion.
TS19: dorsal aorta	Absent		4A
TS19: head mesenchyme	Absent		4A	No detected expression in the head muscles.

Structure	Level	Pattern	Image	Note
TS19: blood vessel	Present	Regionally restricted	4B	Expression in the main veins and arteries in the head.

Structure	Level	Pattern	Image	Note
TS19: blood vessel	Present	Regionally restricted	4C	Expression in the hyaloid plexus and the surrounding vasculature of developing eye.
TS19: cornea epithelium	Present		4C

Structure	Level	Pattern	Image	Note
TS19: nasal pit	Present	Regionally restricted	4D	Expression in the vomeronasal region of the olfactory pit.

Structure	Level	Pattern	Image	Note
TS19: nasal process	Present	Regionally restricted	4E	Expression in the epithelia of the anterior medial nasal process.

Structure	Level	Pattern	Image	Note
TS19: endolymphatic appendage	Present	Regionally restricted	4F	Expression in the lateral part of the endolymphatic diverticular appendage.
TS19: inner ear epithelium	Present	Regionally restricted	4F	Expression in the anterior lining of the otic vesicle.

Structure	Level	Pattern	Image	Note
TS19: endocardial cushion	Present		4G
TS19: endocardial lining	Weak	Regionally restricted	4G	Most of the expression in the endocardium lining of the trabeculation of the heart chambers and the canals is lost.

Structure	Level	Pattern	Image	Note
TS19: myotome	Present	Regionally restricted	4H	Expression in the thoracic precursors from the epaxial to the hypaxial domains.

Structure	Level	Pattern	Image	Note
TS19: myotome	Present	Regionally restricted	4I	Expression can be seen in all muscle fibres, although at more ventral positions it is restricted to the rostral and caudal domains, while absent from central locations.

Structure	Level	Pattern	Image	Note
TS20: skeletal muscle	Present		5A
TS20: cardiovascular system	Absent		5A	No vascular expression, including the heart.
TS20: endolymphatic appendage	Present		5A
TS20: olfactory epithelium	Present	Regionally restricted	5A	Expression in the vomeronasal region of the olfactory pit.

Structure	Level	Pattern	Image	Note
TS20: cornea epithelium	Present		5B

Structure	Level	Pattern	Image	Note
TS20: skeletal muscle	Strong	Regionally restricted	5C	Strong expression in the epaxial muscle.

Structure	Level	Pattern	Image	Note
TS20: skeletal muscle	Strong	Regionally restricted	5D	Strong expression in extensor and flexor muscles in the forelimb.

Structure	Level	Pattern	Image	Note
TS20: skeletal muscle	Strong	Regionally restricted	5E	Strong expression in extensor and flexor muscles.
TS20: radius-ulna pre-cartilage condensation	Present	Regionally restricted	5E	Expression in the growing cones of some cartilage condensations in the forelimb, such as ulna and radius.

Structure	Level	Pattern	Image	Note
TS20: skeletal muscle	Strong	Regionally restricted	5F	Strong expression in extensor and flexor muscles in the hindlimb.

Structure	Level	Pattern	Image	Note
TS20: skeletal muscle	Strong	Regionally restricted	5G	Strong expression in hypaxial derived muscles such as shoulder and intercostal musculature.

Structure	Level	Pattern	Image	Note
TS20: trachea epithelium	Present	Regionally restricted	5H	Expression in the ventral lining of the trachea.

Structure	Level	Pattern	Image	Note
TS22: limb cartilage condensation	Present	Regionally restricted	5I	Expression in the cartilage condensations in the limbs.

Structure	Level	Pattern	Image	Note
TS22: skeletal muscle	Weak