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Gene Expression Data
Assay Details
Assay
Reference: J:129979 Gustavsson P, et al., Increased expression of Grainyhead-like-3 rescues spina bifida in a folate-resistant mouse model. Hum Mol Genet. 2007 Nov 1;16(21):2640-6
Assay type: RT-PCR
MGI Accession ID: MGI:5050748
Gene symbol: Grhl3
Gene name: grainyhead like transcription factor 3
Probe: Grhl3-pG, Grhl3-pH
Probe preparation: labelled with SYBR green
Assay notes: Quantitative real time RT-PCR. The data are shown as a graph relative to Gapdh expression. RNA was extracted from the spinal region of E10.5 embryos caudal to the 20th somite.
Results Image: 2
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex
25-27 somites E9.5 (a) TS15: future spinal cord Present 0.7 µg; total RNA involves: GFF Not Specified
28-29 somites E9.5 (a) TS15: future spinal cord Present 0.7 µg; total RNA involves: GFF Not Specified
30-31 somites E10.5 (a) TS17: future spinal cord Present 0.7 µg; total RNA involves: GFF Not Specified
E11.5 E11.5 (a) TS19: future spinal cord Present 0.7 µg; total RNA involves: GFF Not Specified
25-27 somites E9.5 (a) TS15: future spinal cord Present (b) 0.7 µg; total RNA GFF-Grhl3ct Grhl3ct/Grhl3ctNot Specified
28-29 somites E9.5 (a) TS15: future spinal cord Present (c) 0.7 µg; total RNA GFF-Grhl3ct Grhl3ct/Grhl3ctNot Specified
30-31 somites E10.5 (a) TS17: future spinal cord Present (d) 0.7 µg; total RNA GFF-Grhl3ct Grhl3ct/Grhl3ctNot Specified
E11.5 E11.5 (a) TS19: future spinal cord Present (e) 0.7 µg; total RNA GFF-Grhl3ct Grhl3ct/Grhl3ctNot Specified
Notes:
(a) Age assigned by curator based on morphological criteria supplied by authors.
(b) Expression was similar to wild-type.
(c) Expression is reduced by 56% compared to wild-type.
(d) Expression is reduced by 41% compared to wild-type.
(e) Expression is lower compared to wt.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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