Tg(Thy1-EGFP)498Cnl
Transgene Detail
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| Symbol: |
Tg(Thy1-EGFP)498Cnl |
| Name: |
transgene insertion 498, Christiaan N Levelt |
| MGI ID: |
MGI:5050504 |
| Synonyms: |
Thy1-EGFP-F, TLG 498 |
| Transgene: |
Tg(Thy1-EGFP)498Cnl Location: unknown
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| Alliance: |
Tg(Thy1-EGFP)498Cnl page
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| Transgene Type: |
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Transgenic (Reporter) |
| Mutation: |
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Insertion
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Mutation details: The construct has the sequence of a membrane associated EGFP (EGFP-F) under control of the Thy1 promoter. A transcriptional STOP cassette flanked by loxP sites is inserted between the promoter and EGFP coding region, requiring that cre-mediated excision takes place to activate the reporter. Linearized DNA was injected in pronuclei of C57BL/6 oocytes. Ten lines were created. Founders were crossed to cre-expressing mice to assess expression patterns. Line TLG 498 showed EGFP expression in >90% of pyramidal neurons in the neocortex and hippocampus. This line (first line produced that demonstrated good expression levels) was utilized to obtain most quantitative data presented. Line 1157 (produced at a later time) expressed EGFP-F in all pyramidal neurons and due to higher expression levels was utilized mainly for in vivo and in vitro imaging experiments.
(J:139674)
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| Mouse strains and cell lines
available from the International Mouse Strain Resource
(IMSR) |
| Carrying this Mutation: |
Mouse Strains: 0 strains available
Cell Lines: 0 lines available
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| Original: |
J:139674 Chakravarthy S, et al., Cre-dependent expression of multiple transgenes in isolated neurons of the adult forebrain. PLoS One. 2008;3(8):e3059 |
| All: |
2 reference(s) |
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Analysis Tools
