Tkt Northern blot Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:37310 Sax CM, et al., Transketolase is a major protein in the mouse cornea. J Biol Chem. 1996 Dec 27;271(52):33568-74
Assay type:	Northern blot
MGI Accession ID:	MGI:5050276
Gene symbol:	Tkt
Gene name:	transketolase

Probe:	p71
Probe preparation:	Double stranded labelled with radioactivity DNA
Visualized with:	Autoradiography

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	2.1 kb	Amount	Genetic Background	Mutant Allele(s)	Sex
Embryonic day 17	E17.0 (a)	TS25: eye	Present (b)	5 µg; total RNA	FVB/N		Not Specified
Newborn	P newborn	TS27: eye	Present (b)	5 µg; total RNA	FVB/N		Not Specified
15 Days	P15	TS28: eye	Present (c)	5 µg; total RNA	FVB/N		Not Specified
7 days	P7	TS28: cornea	Weak	5 µg; total RNA	FVB/N		Not Specified
15 days	P15	TS28: cornea	Present (d)	5 µg; total RNA	FVB/N		Not Specified
8 Weeks	P w 8	TS28: cornea	Present (e)	5 µg; total RNA	FVB/N		Not Specified

Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Low and nearly equal levels in E17 and newborn whole eyes.
(c) 2-fold higher levels
(d) 5-fold increase
(e) 2-fold higher levels as compared to 15-day levels.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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