Summary | Mutation origin | Mutation description | Recombinase activity | Phenotypes | Find Mice (IMSR) | References

Symbol:	Pou5f1tm1.1(cre/Esr1*)Yseg
Name:	POU domain, class 5, transcription factor 1; targeted mutation 1.1, Yoav Segal
MGI ID:	MGI:5049897
Synonyms:	Oct4CreERT2, Oct4-MerCreMer
Gene:	Pou5f1  Location: Chr17:35816929-35821674 bp, + strand  Genetic Position: Chr17, 18.69 cM
Alliance:	Pou5f1tm1.1(cre/Esr1*)Yseg page

Germline Transmission:	Earliest citation of germline transmission: J:194647
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	C57BL/6

Allele Type:    Targeted (Inducible, Recombinase) Inducer:    tamoxifen Mutation:    Insertion   Pou5f1tm1.1(cre/Esr1*)Yseg expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Pou5f1 (MGI:101893)   Mutation details: A targeting vector containing an FRT-site flanked NEO cassette and IRES-MerCreMer sequence was inserted in the 3' UTR of Oct4/Pou5f1 locus. The construct was electroporated into C57BL/6 derived embryonic stem (ES) cells from inGenious Targeting Laboratory. Correctly targeted ES cells were injected into recipient B6(Cg)-Tyrc-2J/J or BALB/c blastocysts. The resulting chimeric animals were crossed to B6.Cg-Tg(ACTFLPe)9205Dym/J mice to remove the FRT site flanked selection cassette. (J:194647)

Activity:	Tissue activity of this recombinase allele
Driver:	Pou5f1 (mouse) Summary of all recombinase alleles driven by Pou5f1. Alliance mouse Pou5f1 gene page

View phenotypes and curated references for all genotypes (concatenated display).

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Pou5f1 Mutation:	82 strains or lines available

Original:	J:194647 Greder LV, et al., Analysis of endogenous Oct4 activation during induced pluripotent stem cell reprogramming using an inducible Oct4 lineage label. Stem Cells. 2012 Nov;30(11):2596-601
All:	16 reference(s)