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Gene Expression Data
Assay Details
Assay
Reference: J:147577 Takahashi N, et al., Deletion of Gtl2, imprinted non-coding RNA, with its differentially methylated region induces lethal parent-origin-dependent defects in mice. Hum Mol Genet. 2009 May 15;18(10):1879-88
Assay type: RT-PCR
MGI Accession ID: MGI:5014276
Gene symbol: Meg3
Gene name: maternally expressed 3
Probe: Gtl2-pI, Gtl2-pJ
Probe preparation: labelled with SYBR green
Assay notes: Quantitative real-time RT-PCR assay. Graphical representation of the expression level relative to that of GAPDH expression.
Results Image: 3B Gtl2
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
B E15.5 (a) TS23: brain Present 0.5 µg; total RNA Not Specified Not Specified
B E15.5 (a) TS23: brain Absent 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3+Not Specified Maternal-KO
B E15.5 (a) TS23: brain Present (b) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3+Not Specified Paternal-KO
B E15.5 (a) TS23: brain Absent 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3tm1KonoNot Specified
T E15.5 (a) TS23: tongue Present 0.5 µg; total RNA Not Specified Not Specified
T E15.5 (a) TS23: tongue Absent 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3+Not Specified Maternal-KO
T E15.5 (a) TS23: tongue Present (b) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3+Not Specified Paternal-KO
T E15.5 (a) TS23: tongue Absent 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3tm1KonoNot Specified
Lu E15.5 (a) TS23: lung Present 0.5 µg; total RNA Not Specified Not Specified
Lu E15.5 (a) TS23: lung Absent 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3+Not Specified Maternal-KO
Lu E15.5 (a) TS23: lung Present (b) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3+Not Specified Paternal-KO
Lu E15.5 (a) TS23: lung Absent 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3tm1KonoNot Specified
Li E15.5 (a) TS23: liver Present 0.5 µg; total RNA Not Specified Not Specified
Li E15.5 (a) TS23: liver Absent 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3+Not Specified Maternal-KO
Li E15.5 (a) TS23: liver Present (b) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3+Not Specified Paternal-KO
Li E15.5 (a) TS23: liver Absent 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3tm1KonoNot Specified
P E15.5 (a) TS28: placenta Present 0.5 µg; total RNA Not Specified Not Specified
P E15.5 (a) TS28: placenta Absent 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3+Not Specified Maternal-KO
P E15.5 (a) TS28: placenta Present (b) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3+Not Specified Paternal-KO
P E15.5 (a) TS28: placenta Absent 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3tm1KonoNot Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) Expression was similar as in wild-type.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory