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Gene Expression Data
Assay Details
Assay
Reference: J:147577 Takahashi N, et al., Deletion of Gtl2, imprinted non-coding RNA, with its differentially methylated region induces lethal parent-origin-dependent defects in mice. Hum Mol Genet. 2009 May 15;18(10):1879-88
Assay type: RT-PCR
MGI Accession ID: MGI:5014262
Gene symbol: Dlk1
Gene name: delta like non-canonical Notch ligand 1
Probe: Dlk1-pG, Dlk1-pH
Probe preparation: labelled with SYBR green
Assay notes: Quantitative real-time RT-PCR assay. Graphical representation of the expression level relative to that of GAPDH expression.
Results Image: 3B Dlk1
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Amount Genetic BackgroundMutant Allele(s)Sex Note
B E15.5 (a) TS23: brain Present 0.5 µg; total RNA Not Specified Not Specified
B E15.5 (a) TS23: brain Present (b) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3+Not Specified Maternal-KO
B E15.5 (a) TS23: brain Present (c) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3+Not Specified Paternal-KO
B E15.5 (a) TS23: brain Present (d) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3tm1KonoNot Specified
T E15.5 (a) TS23: tongue Present 0.5 µg; total RNA Not Specified Not Specified
T E15.5 (a) TS23: tongue Present (b) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3+Not Specified Maternal-KO
T E15.5 (a) TS23: tongue Present (c) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3+Not Specified Paternal-KO
T E15.5 (a) TS23: tongue Present (d) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3tm1KonoNot Specified
Lu E15.5 (a) TS23: lung Present 0.5 µg; total RNA Not Specified Not Specified
Lu E15.5 (a) TS23: lung Present (b) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3+Not Specified Maternal-KO
Lu E15.5 (a) TS23: lung Present (c) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3+Not Specified Paternal-KO
Lu E15.5 (a) TS23: lung Present (d) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3tm1KonoNot Specified
Li E15.5 (a) TS23: liver Present 0.5 µg; total RNA Not Specified Not Specified
Li E15.5 (a) TS23: liver Present (b) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3+Not Specified Maternal-KO
Li E15.5 (a) TS23: liver Present (c) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3+Not Specified Paternal-KO
Li E15.5 (a) TS23: liver Present (d) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3tm1KonoNot Specified
P E15.5 (a) TS28: placenta Present 0.5 µg; total RNA Not Specified Not Specified
P E15.5 (a) TS28: placenta Present (b) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3+Not Specified Maternal-KO
P E15.5 (a) TS28: placenta Present (c) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3+Not Specified Paternal-KO
P E15.5 (a) TS28: placenta Present (d) 0.5 µg; total RNA involves: 129P2/OlaHsd * C57BL/6N * DBA/2 Meg3tm1Kono/Meg3tm1KonoNot Specified
Notes:
(a) Age of embryo at noon of plug day not specified in reference.
(b) There was no significant change in the expression of paternally expressed Dlk1 in the Maternal-KO mutant compared to wild-type.
(c) Expression was decreased in the Paternal-KO mutant compared to wild-type.
(d) Expression was decreased compared to wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
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