Summary | Mutation origin | Mutation description | Recombinase activity | Find Mice (IMSR) | References

Symbol:	Otoftm1.1(cre)Mull
Name:	otoferlin; targeted mutation 1.1, Ulrich Mueller
MGI ID:	MGI:5014098
Synonyms:	Otof-Cre
Gene:	Otof  Location: Chr5:30524406-30619276 bp, - strand  Genetic Position: Chr5, 16.48 cM, cytoband B1
Alliance:	Otoftm1.1(cre)Mull page

Germline Transmission:	Earliest citation of germline transmission: J:151755
Parent Cell Line:	Bruce 4 (ES Cell)
Strain of Origin:	B6.Cg-Thy1a
Project Collection:	Neuroscience Blueprint cre

Allele Type:    Targeted (Recombinase) Mutation:    Insertion   Otoftm1.1(cre)Mull expression driven by 1 gene Knock-in expression driven by: Organism Driver Gene Note mouse Otof (MGI:1891247)   Mutation details: A targeting vector was designed to insert a nuclear-localized Cre recombinase gene and polyA signal followed by an FRT-flanked PGK-Neo cassette into the initiation codon of the otoferlin (Otof) locus. This construct was electroporated into C57BL/6-derived Bruce4 embryonic stem (ES) cells. Chimeric mice were bred to C57BL/6 mice to establish the colony. Next, mutant mice were bred with FLPe-expressing mice on a C57BL/6 congenic background to remove the FRT-flanked PGK-Neo cassette. The resulting Otof-Cre mice were then bred to C57BL/6J inbred mice for approximately two generations (selecting away from the FLPe transgene). (J:151755)

Activity:	Tissue activity of this recombinase allele
Driver:	Otof (mouse) Summary of all recombinase alleles driven by Otof. Comparative matrix view of recombinase activities Alliance mouse Otof gene page

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Otof Mutation:	108 strains or lines available

Original:	J:151755 NIH Neuroscience Blueprint Cre Driver Network, Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network. MGI Direct Data Submission. 2009;
All:	8 reference(s)