Ccktm1.1(cre)Zjh
Targeted Allele Detail
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Symbol: |
Ccktm1.1(cre)Zjh |
Name: |
cholecystokinin; targeted mutation 1.1, Z Josh Huang |
MGI ID: |
MGI:5014096 |
Synonyms: |
Cck-IRES-Cre |
Gene: |
Cck Location: Chr9:121318890-121324728 bp, - strand Genetic Position: Chr9, 72.43 cM, cytoband F4
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Alliance: |
Ccktm1.1(cre)Zjh page
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Germline Transmission: |
Earliest citation of germline transmission:
J:151755
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Parent Cell Line: |
v6.5 (ES Cell)
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Strain of Origin: |
(C57BL/6 x 129S4/SvJae)F1
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Project Collection: |
Neuroscience Blueprint cre
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Allele Type: |
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Targeted (Recombinase) |
Mutation: |
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Insertion
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Ccktm1.1(cre)Zjh expression driven by
1 gene
Knock-in expression driven by:
Organism |
Driver Gene |
Note |
mouse |
Cck (MGI:88297) |
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Mutation details: A targeting vector was designed to insert an internal ribosome entry site (IRES), a cre recombinase sequence, a polyA sequence, and an frt-flanked neo cassette into the 3' untranslated region (after the translational termination site) of the cholecystokinin locus (Cck). This construct was electroporated into C57BL/6;129 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. Chimeric males were bred with Actin-FLPe females (on a C57BL/6 congenic background) to achieve germline transmission and to remove the neo selection cassette. The resulting pups harboring the cre targeted mutation were selected. These Cck-IRES-Cre mice were subsequently bred together for several generations (and the FLPe transgene was removed).
(J:151755)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Cck
(mouse)
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Original: |
J:151755 NIH Neuroscience Blueprint Cre Driver Network, Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network. MGI Direct Data Submission. 2009; |
All: |
87 reference(s) |
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