Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(CD4-HIV)#gPjo
Name:	transgene insertion #g, Paul Jolicoeur
MGI ID:	MGI:5008256
Synonyms:	CD4C/HIVNef
Transgene:	Tg(CD4-HIV)#gPjo  Location: unknown
Alliance:	Tg(CD4-HIV)#gPjo page

Strain of Origin:

(C57BL/6 x C3H)F2

Transgene Type:    Transgenic (Inserted expressed sequence) Mutation:    Insertion   Mutation details: Mouse CD4 enhancer sequences, the human CD4 promoter, 8.8 kb of HIV sequences, and an SV40 polyadenylation signal was used for the transgene. The enhancer and promoter sequences were used to drive expression in CD4+ T cells and cells of the dendritic/macrophage lineage. The HIV sequence contained the following mutations: stop oligos (a 14-mer sequence introdicing stop codons in all 3 reading frames) in the gag, vif, rev, and env genes, a 22 bp deletion of the pol gene followed by a stop oligo, a deletion of nucleotides 5622-5737 in the vpr gene, a deletion of nucleotides 6062-6180 in the vpu gene, and a G to T nonsense mutation atnucleotide 5854 of the tat gene. Northern blot analysis showed high transgene expression in the thymus, moderate expression in lymph nodes, and weak expression in kidneys, lungs, intestines, and liver. The pound symbol (#g) corresponds to the unnamed and/or pooled lines with only the nef gene intact (F26990, F27011, F27367, and F27372). (J:78756)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available

Original:	J:170500 Bouchet J, et al., Inhibition of the Nef regulatory protein of HIV-1 by a single-domain antibody. Blood. 2011 Mar 31;117(13):3559-68
All:	3 reference(s)