Summary | Transgene origin | Transgene description | Find Mice (IMSR) | References

Symbol:	Tg(Tph2-COP4*H134R/EYFP)5Gfng
Name:	transgene insertion 5, Guoping Feng
MGI ID:	MGI:4950484
Synonyms:	TpH2-ChR2-YFP line 5
Transgene:	Tg(Tph2-COP4*H134R/EYFP)5Gfng  Location: unknown
Alliance:	Tg(Tph2-COP4*H134R/EYFP)5Gfng page

Strain of Origin:

(C57BL/6 x SJL)F1

Transgene Type:    Transgenic (Reporter) Mutation:    Insertion   Mutation details: Transgene expression of the mhChR2::YFP fusion protein is directed to serotonergic neuronal populations by the mouse tryptophan hydroxylase 2 (Tph2 or TpH2) promoter/enhancer regions on the BAC transgene. The mhChR2::YFP fusion protein is composed of a mammalian codon-optimized Chlamydomonas reinhardtii-derived channelrhodopsin-2 that was modified to harbor a gain-of-function H134R substitution (mhChR2; also called hChR2-H134R) fused in-frame with an enhanced yellow fluorescent protein (EYFP). The mhChR2 is designed to cause larger stationary photocurrents compared to ChR2. The bacterial opsins are retinal-binding proteins that provide light-dependent ion transport and sensory functions to a family of halophilic bacteria; and this mhChR2 functions as a blue light-driven cation channel that depolarizes the cell and causes action potentials. As such, illuminating mhChR2-expressing neurons with blue light (450-490 nm) leads to rapid and reversible photostimulation of action potential firing/neural activity in these cells. EYFP expression is visible by direct fluorescence (epifluorescence microscope). TpH2-mhChR2-YFP mice derived from founder line 5 exhibit medium EYFP expression in dorsal raphe nucleus, gigantocellular reticular nucleus, paramedian reticular nucleus, and other serotonergic nuclei. Anti-TpH2 immunofluorescent signal co-localizes specifically with anti-YFP antibody-labeled neurons in the cell types listed above; suggesting mhChR2-EYFP labels all the serotonergic neurons with no ectopic expression. No tissues other than brain were tested for EYFP expression. (J:171571)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available

Original:	J:171571 Feng G, Direct Data Submission 2011/05/16. MGI Direct Data Submission. 2011;
All:	6 reference(s)