Probe:	3-OST-1 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS24: cerebral cortex ventricular layer	Present		2A
TS24: cortical plate	Present		2A
TS24: cerebral cortex intermediate zone	Weak		2A

Structure	Level	Pattern	Image	Note
TS24: cortical plate	Present		2D

Structure	Level	Pattern	Image	Note
TS27: cerebral cortex	Present	Regionally restricted	3A	Expression was detected in most regions of the ventricular zone, the cortical plate, layers V/VI and the intermediate zone. Signal in the cortical plate was stronger toward the marginal zone and gradually weakened toward the intermediate zone.
TS27: cortical layer V	Present		3A
TS27: cortical layer VI	Present		3A

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present	Uniform	3E
TS28: cortical layer III	Present	Uniform	3E
TS28: cortical layer IV	Present	Uniform	3E
TS28: cortical layer V	Present	Uniform	3E
TS28: cortical layer VI	Present	Uniform	3E

Structure	Level	Pattern	Image	Note
TS28: cortical layer II	Present	Uniform	3I
TS28: cortical layer III	Present	Uniform	3I
TS28: cortical layer IV	Present	Uniform	3I
TS28: cortical layer V	Present	Uniform	3I
TS28: cortical layer VI	Present	Uniform	3I

Structure	Level	Pattern	Image	Note
TS28: cortical layer V	Weak	Regionally restricted	5F , 5C	Strong signals were detected in the pyramidal neurons in layer V.

Structure	Level	Pattern	Image	Note
TS27: cerebellum Purkinje cell layer	Strong
TS27: cerebellum external granule cell layer	Strong

Structure	Level	Pattern	Image	Note
TS28: cerebellum external granule cell layer	Weak

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present
TS28: cerebellum internal granule cell layer	Present

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present
TS28: cerebellum granule cell layer	Present
TS28: cerebellum molecular layer	Weak