Gene Expression Data
Assay Details
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Assay
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Reference:
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J:170598
Coy S, et al., A Novel Gli3 Enhancer Controls the Gli3 Spatiotemporal Expression Pattern through a TALE Homeodomain Protein Binding Site. Mol Cell Biol. 2011 Apr;31(7):1432-43
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:4949499
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Gene symbol:
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Gli3
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Gene name:
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GLI-Kruppel family member GLI3
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Probe:
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Gli3 cDNA8
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
5A:
embryonic day 10.5
(more  )
Specimen
5A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
5A:
embryonic day 10.5
(more )
Specimen
5A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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Specimen
5B:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Pbx1tm1Mlc/Pbx1tm1Mlc
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Present |
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5B left
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Although overall expression is unaffected, expression is strongly upregulated in the rostral neural tube of the mutants.
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Specimen
5B:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac
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Mutant Allele(s):
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Pbx1tm1Mlc/Pbx1tm1Mlc
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Age:
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embryonic day 10.5
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Agarose
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: neural tube |
Present |
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5B right
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Although overall expression is unaffected, expression is strongly upregulated in the rostral neural tube of the mutants.
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| TS17: neural tube floor plate |
Present |
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5B right
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Analysis Tools
